Anti-ZIC3 Antibody (CAB9476)
- SKU:
- CAB9476
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| 抗体名: | Anti-ZIC3 Antibody |
| 抗体コード: | CAB9476 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZIC3 (NP_003404.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, Mouse brain, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZIC3 (NP_003404.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MTML LDGG PQFP GLGV GSFG APRH HEMP NREP AGMG LNPF GDST HAAA AAAA AAAF KLSP AAAH DLSS GQSS AFTP QGSG YANA LGHH HHHH HHHH HTSQ VPSY GGAA SAAF NSTR EFLF RQRS SGLS EAAS GGGQ HGLF AGSA SSLH APAG IPEP PSYL LFPG LHEQ GAGH PSPT GHVD NNQV HLGL RGEL FGRA DPYR PVAS PRTD PYAA GAQF PNYS PMNM NMGV NVAA HHGP GAFF |
| 遺伝子ID: | 7547 |
| Uniprot: | O60481 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 50kDa |
| 観察された分子量: | 51kDa |
| 同義語: | ZIC3, HTX, HTX1, VACTERLX, ZNF203 |
| バックグラウンド: | This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs. |
| UniProt Protein Function: | ZIC3: Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. Defects in ZIC3 are the cause of visceral heterotaxy X- linked type 1 (HTX1). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy X- linked type 1 include dextrocardia, corrected transposition of great arteries, ventricular septal defect, patent ductus arteriosus, pulmonic stenosis, situs inversus viscerum, and asplenia and/or polysplenia. Defects in ZIC3 are a cause of VACTERL association X- linked with or without hydrocephalus (VACTERLX). A syndrome characterized by vertebral anomalies, anal atresia, cardiac malformations, tracheoesophageal fistula, renal anomalies (urethral atresia with hydronephrosis), and limb anomalies (hexadactyly, humeral hypoplasia, radial aplasia, and proximally placed thumb). Some patients may have hydrocephalus. Some cases of VACTERL-H are associated with increased chromosome breakage and rearrangement. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; C2H2-type zinc finger protein; Transcription factor Chromosomal Location of Human Ortholog: Xq26.2 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding; sequence-specific DNA binding; transcription factor activity Biological Process: determination of left/right symmetry; heart looping; lung development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; somatic stem cell maintenance Disease: Heterotaxy, Visceral, 1, X-linked; Vacterl Association, X-linked, With Or Without Hydrocephalus |
| NCBI Summary: | This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O60481 |
| NCBI GenInfo Identifier: | 6137314 |
| NCBI Gene ID: | 7547 |
| NCBI Accession: | O60481.1 |
| UniProt Secondary Accession: | O60481,Q14DE5, Q5JY75, B2CNW4, |
| UniProt Related Accession: | O60481 |
| Molecular Weight: | 50,045 Da |
| NCBI Full Name: | Zinc finger protein ZIC 3 |
| NCBI Synonym Full Names: | Zic family member 3 |
| NCBI Official Symbol: | ZIC3 |
| NCBI Official Synonym Symbols: | HTX; HTX1; ZNF203; VACTERLX |
| NCBI Protein Information: | zinc finger protein ZIC 3 |
| UniProt Protein Name: | Zinc finger protein ZIC 3 |
| UniProt Synonym Protein Names: | Zinc finger protein 203; Zinc finger protein of the cerebellum 3 |
| Protein Family: | Zinc finger protein |
| UniProt Gene Name: | ZIC3 |
| UniProt Entry Name: | ZIC3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ZIC3 antibody (CAB9476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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