Anti-UFD1L Antibody (CAB3255)
- SKU:
- CAB3255
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-UFD1L Antibody |
| 抗体コード: | CAB3255 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human UFD1L (NP_005650.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, A-431, Mouse heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human UFD1L (NP_005650.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MFSF NMFD HPIP RVFQ NRFS TQYR CFSV SMLA GPND RSDV EKGG KIIM PPSA LDQL SRLN ITYP MLFK LTNK NSDR MTHC GVLE FVAD EGIC YLPH WMMQ NLLL EEGG LVQV ESVN LQVA TYSK FQPQ SPDF LDIT NPKA VLEN ALRN FACL TTGD VIAI NYNE KIYE LRVM ETKP DKAV SIIE CDMN VDFD APLG YKEP ERQV QHEE STEG EADH SGYA GELG FRAF SGSG NRLD GKKK GVEP SPSP IKPG DIKR GIPN YEFK LGKI TFIR NSRP LVKK VEED EAGG RFVA FSGE GQSL RKKG RKP |
| 遺伝子ID: | 7353 |
| Uniprot: | Q92890 |
| セルラーロケーション: | Cytoplasm, Nucleus, cytosol |
| 計算された分子量: | 29kDa/34kDa/38kDa |
| 観察された分子量: | 37kDa |
| 同義語: | UFD1, UFD1L |
| バックグラウンド: | The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18. |
| UniProt Protein Function: | UFD1L: Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures. Belongs to the UFD1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin-specific protease; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: cytosol; nucleus Molecular Function:protein binding; ubiquitin-specific protease activity; protein complex binding; receptor binding Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; skeletal development |
| NCBI Summary: | The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Jun 2009] |
| UniProt Code: | Q92890 |
| NCBI GenInfo Identifier: | 160332310 |
| NCBI Gene ID: | 7353 |
| NCBI Accession: | Q92890.3 |
| UniProt Secondary Accession: | Q92890,Q9Y5N0, A8MW31, |
| UniProt Related Accession: | Q92890 |
| Molecular Weight: | 307 |
| NCBI Full Name: | Ubiquitin fusion degradation protein 1 homolog |
| NCBI Synonym Full Names: | ubiquitin fusion degradation 1 like (yeast) |
| NCBI Official Symbol: | UFD1L |
| NCBI Official Synonym Symbols: | UFD1 |
| NCBI Protein Information: | ubiquitin fusion degradation protein 1 homolog; UB fusion protein 1 |
| UniProt Protein Name: | Ubiquitin fusion degradation protein 1 homolog |
| UniProt Gene Name: | UFD1L |
| UniProt Entry Name: | UFD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UFD1L antibody (CAB3255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon using UFD1L antibody (CAB3255) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using UFD1L antibody (CAB3255). |
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