Anti-UBE2D4 Antibody (CAB8332)
- SKU:
- CAB8332
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-UBE2D4 Antibody |
| 抗体コード: | CAB8332 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D4 (NP_057067.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | BT-474, HepG2, MCF7, Mouse liver, Mouse skeletal muscle, Mouse kidney, Rat lung, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D4 (NP_057067.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MALK RIQK ELTD LQRD PPAQ CSAG PVGD DLFH WQAT IMGP NDSP YQGG VFFL TIHF PTDY PFKP PKVA FTTK IYHP NINS NGSI CLDI LRSQ WSPA LTVS KVLL SICS LLCD PNPD DPLV PEIA HTYK ADRE KYNR LARE WTQK YAM |
| 遺伝子ID: | 51619 |
| Uniprot: | Q9Y2X8 |
| セルラーロケーション: | |
| 計算された分子量: | 16kDa |
| 観察された分子量: | 17kDa |
| 同義語: | UBE2D4, HBUCE1 |
| バックグラウンド: |
| UniProt Protein Function: | UBE2D4: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Ligase Chromosomal Location of Human Ortholog: 7p13 Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: protein ubiquitination |
| UniProt Code: | Q9Y2X8 |
| NCBI GenInfo Identifier: | 74753478 |
| NCBI Gene ID: | 51619 |
| NCBI Accession: | Q9Y2X8.1 |
| UniProt Secondary Accession: | Q9Y2X8,A4D1V0, |
| UniProt Related Accession: | Q9Y2X8 |
| Molecular Weight: | 16,649 Da |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 D4 |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 D4 (putative) |
| NCBI Official Symbol: | UBE2D4 |
| NCBI Official Synonym Symbols: | HBUCE1 |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 D4 |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 D4 |
| UniProt Synonym Protein Names: | E2 ubiquitin-conjugating enzyme D4; HBUCE1; Ubiquitin carrier protein D4; Ubiquitin-protein ligase D4 |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2D4 |
| UniProt Entry Name: | UB2D4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBE2D4 antibody (CAB8332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
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