Anti-UBE2D2 Antibody (CAB10818)
- SKU:
- CAB10818
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-UBE2D2 Antibody |
| 抗体コード: | CAB10818 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D2 (NP_003330.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, K-562, 293T, Jurkat, Mouse testis, Mouse brain, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D2 (NP_003330.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MALK RIHK ELND LARD PPAQ CSAG PVGD DMFH WQAT IMGP NDSP YQGG VFFL TIHF PTDY PFKP PKVA FTTR IYHP NINS NGSI CLDI LRSQ WSPA LTIS KVLL SICS LLCD PNPD DPLV PEIA RIYK TDRE KYNR IARE WTQK YAM |
| 遺伝子ID: | 7322 |
| Uniprot: | P62837 |
| セルラーロケーション: | |
| 計算された分子量: | 13kDa/16kDa |
| 観察された分子量: | 17kDa |
| 同義語: | UBE2D2, E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B |
| バックグラウンド: | Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. |
| UniProt Protein Function: | UBE2D2: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3. Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; EC 6.3.2.19; Ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 5q31.2 Cellular Component: cytosol; nucleoplasm; protein complex; ubiquitin ligase complex Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: protein autoubiquitination; protein modification process; protein polyubiquitination; protein ubiquitination; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; ubiquitin-dependent protein catabolic process |
| NCBI Summary: | Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013] |
| UniProt Code: | P62837 |
| NCBI GenInfo Identifier: | 51338685 |
| NCBI Gene ID: | 7322 |
| NCBI Accession: | P62837.1 |
| UniProt Secondary Accession: | P62837,P51669, Q3MN78, Q96RP6, D3DQC9, |
| UniProt Related Accession: | P62837 |
| Molecular Weight: | 13,635 Da |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 D2 |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 D2 |
| NCBI Official Symbol: | UBE2D2 |
| NCBI Official Synonym Symbols: | UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2 |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 D2 |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 D2 |
| UniProt Synonym Protein Names: | (E3-independent) E2 ubiquitin-conjugating enzyme D2 (EC:2.3.2.24 |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2D2 |
| UniProt Entry Name: | UB2D2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBE2D2 antibody (CAB10818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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