Anti-TRPM8 Antibody (CAB12572)
- SKU:
- CAB12572
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-TRPM8 Antibody |
| 抗体コード: | CAB12572 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 880-960 of human TRPM8 (NP_076985.4). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | LO2, 293T, Mouse kidney, Mouse heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 880-960 of human TRPM8 (NP_076985.4). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AFGV ARQG ILRQ NEQR WRWI FRSV IYEP YLAM FGQV PSDV DGTT YDFA HCTF TGNE SKPL CVEL DEHN LPRF PEWI TIPL V |
| 遺伝子ID: | 79054 |
| Uniprot: | Q7Z2W7 |
| セルラーロケーション: | Cell membrane, Endoplasmic reticulum membrane, Membrane raft, Multi-pass membrane protein |
| 計算された分子量: | 5kDa/18kDa/79kDa/127kDa |
| 観察された分子量: | 122kDa |
| 同義語: | TRPM8, LTRPC6, TRPP8 |
| バックグラウンド: |
| UniProt Protein Function: | TRPM8: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Channel, cation; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 2q37.1 Cellular Component: plasma membrane Molecular Function:calcium channel activity; protein binding |
| UniProt Code: | Q7Z2W7 |
| NCBI GenInfo Identifier: | 143811469 |
| NCBI Gene ID: | 79054 |
| NCBI Accession: | Q7Z2W7.2 |
| UniProt Secondary Accession: | Q7Z2W7,Q3YFM7, Q6QNH9, Q8TAC3, Q8TDX8, Q9BVK1, A0AVG2 |
| UniProt Related Accession: | Q7Z2W7 |
| Molecular Weight: | 79,096 Da |
| NCBI Full Name: | Transient receptor potential cation channel subfamily M member 8 |
| NCBI Synonym Full Names: | transient receptor potential cation channel subfamily M member 8 |
| NCBI Official Symbol: | TRPM8 |
| NCBI Official Synonym Symbols: | TRPP8; LTRPC6 |
| NCBI Protein Information: | transient receptor potential cation channel subfamily M member 8 |
| UniProt Protein Name: | Transient receptor potential cation channel subfamily M member 8 |
| UniProt Synonym Protein Names: | Long transient receptor potential channel 6; LTrpC-6; LTrpC6; Transient receptor potential p8; Trp-p8 |
| Protein Family: | TRPM8 channel-associated factor |
| UniProt Gene Name: | TRPM8 |
| UniProt Entry Name: | TRPM8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TRPM8 antibody (CAB12572) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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