Anti-TOMM40 Antibody (CAB3213)

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SKU:
CAB3213
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
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Description

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抗体名:Anti-TOMM40 Antibody
抗体コード:CAB3213
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC IF
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human TOMM40 (NP_001122389.1).
申し込み:WB IHC IF
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:Human, Mouse, Rat
ポジティブサンプル:U-2OS, HepG2, Mouse brain, Mouse testis, Rat brain
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human TOMM40 (NP_001122389.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MGNV LAAS SPPA GPPP PPAP ALVG LPPP PPSP PGFT LPPL GGSL GAGT STSR SSER TPGA ATAS ASGA AEDG ACGC LPNP GTFE ECHR KC
遺伝子ID:10452
Uniprot:O96008
セルラーロケーション:Mitochondrion outer membrane, Multi-pass membrane protein
計算された分子量:34kDa/37kDa
観察された分子量:38kDa
同義語:TOMM40, C19orf1, D19S1177E, PER-EC1, PEREC1, TOM40
バックグラウンド:The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:TOMM40: Channel-forming protein essential for import of protein precursors into mitochondria. Belongs to the Tom40 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Mitochondrial

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: nucleoplasm; pore complex; mitochondrial outer membrane translocase complex; mitochondrion; cytoplasm; integral to membrane; integral to mitochondrial outer membrane

Molecular Function:protein transmembrane transporter activity; porin activity

Biological Process: cellular protein metabolic process; ion transport; protein targeting to mitochondrion

NCBI Summary:The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]
UniProt Code:O96008
NCBI GenInfo Identifier:12230369
NCBI Gene ID:10452
NCBI Accession:O96008.1
UniProt Related Accession:O96008
Molecular Weight:Observed MW: 38kDa
NCBI Full Name:Mitochondrial import receptor subunit TOM40 homolog
NCBI Synonym Full Names:translocase of outer mitochondrial membrane 40
NCBI Official Symbol:TOMM40  
NCBI Official Synonym Symbols:TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E  
NCBI Protein Information:mitochondrial import receptor subunit TOM40 homolog
UniProt Protein Name:Mitochondrial import receptor subunit TOM40 homolog
UniProt Synonym Protein Names:Protein Haymaker; Translocase of outer membrane 40 kDa subunit homolog; p38.5
Protein Family:Mitochondrial import receptor
UniProt Gene Name:TOMM40  
UniProt Entry Name:TOM40_HUMAN
Anti-TOMM40 Antibody (CAB3213)Western blot analysis of extracts of various cell lines, using TOMM40 antibody (CAB3213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-TOMM40 Antibody (CAB3213)Confocal immunofluorescence analysis of U2OS cells using TOMM40 Polyclonal Antibody (CAB3213) at dilution of 1:200. Blue: DAPI for nuclear staining.
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