Anti-SCNN1A Antibody (CAB5538)
- SKU:
- CAB5538
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-SCNN1A Antibody |
| 抗体コード: | CAB5538 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 105-225 of human SCNN1A (NP_001029.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse lung, Mouse intestine |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 105-225 of human SCNN1A (NP_001029.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LFGE YFSY PVSL NINL NSDK LVFP AVTI CTLN PYRY PEIK EELE ELDR ITEQ TLFD LYKY SSFT TLVA GSRS RRDL RGTL PHPL QRLR VPPP PHGA RRAR SVAS SLRD NNPQ VDWK DWKI G |
| 遺伝子ID: | 6337 |
| Uniprot: | P37088 |
| セルラーロケーション: | Apical cell membrane, Cell projection, Multi-pass membrane protein, cilium |
| 計算された分子量: | 28kDa/73kDa/75kDa/77kDa/78kDa/81kDa |
| 観察された分子量: | 76kDa |
| 同義語: | SCNN1A, BESC2, ENaCa, ENaCalpha, SCNEA, SCNN1 |
| バックグラウンド: | Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| UniProt Protein Function: | ENaC-alpha: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. By aldosterone. Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. Activated by WNK1, WNK2, WNK3 and WNK4. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1A subfamily. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transporter, ion channel; Membrane protein, multi-pass; Channel, sodium; Membrane protein, integral Chromosomal Location of Human Ortholog: 12p13 Cellular Component: apical plasma membrane; integral to plasma membrane; plasma membrane Molecular Function:amiloride-sensitive sodium channel activity; protein binding; WW domain binding Biological Process: multicellular organismal water homeostasis; sodium ion homeostasis Disease: Bronchiectasis With Or Without Elevated Sweat Chloride 2; Pseudohypoaldosteronism, Type I, Autosomal Recessive |
| NCBI Summary: | Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009] |
| UniProt Code: | P37088 |
| NCBI GenInfo Identifier: | 585966 |
| NCBI Gene ID: | 6337 |
| NCBI Accession: | P37088.1 |
| UniProt Secondary Accession: | P37088,O43271, Q6GSQ6, Q9UM64, A5X2U9, B4E2Q5, C5HTZ0 |
| UniProt Related Accession: | P37088 |
| Molecular Weight: | |
| NCBI Full Name: | Amiloride-sensitive sodium channel subunit alpha |
| NCBI Synonym Full Names: | sodium channel epithelial 1 alpha subunit |
| NCBI Official Symbol: | SCNN1A |
| NCBI Official Synonym Symbols: | BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha |
| NCBI Protein Information: | amiloride-sensitive sodium channel subunit alpha |
| UniProt Protein Name: | Amiloride-sensitive sodium channel subunit alpha |
| UniProt Synonym Protein Names: | Alpha-NaCH; Epithelial Na(+) channel subunit alpha; Alpha-ENaC; ENaCA; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA |
| Protein Family: | Amiloride-sensitive sodium channel |
| UniProt Gene Name: | SCNN1A |
| UniProt Entry Name: | SCNNA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SCNN1A antibody (CAB5538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of human lung cancer cells using SCNN1A antibody (CAB5538) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of rat lung cells using SCNN1A antibody (CAB5538) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse kidney cells using SCNN1A antibody (CAB5538) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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