Anti-RASAL1 Antibody (CAB15114)
- SKU:
- CAB15114
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| 抗体名: | Anti-RASAL1 Antibody |
| 抗体コード: | CAB15114 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-340 of human RASAL1 (NP_004649.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | A549, MCF7, mouse brain, rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-340 of human RASAL1 (NP_004649.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ICLS VQML EDGQ GRCL RCHV LQAR DLAP RDIS GTSD PFAR VFWG SQSL ETST IKKT RFPH WDEV LELR EMPG APSP LRVE LWDW DMVG KNDF LGMV EFSP KTLQ QKPP KGWF RLLP FPRA EEDS GGNL GALR VKVR LIED RVLP SQCY QPLM ELLM ESVQ GPAE EDTA SPLA LLEE LTLG DCRQ DLAT KLVK LFLG RGLA GRFL DYLT RREV ARTM DPNT L |
| 遺伝子ID: | 8437 |
| Uniprot: | O95294 |
| セルラーロケーション: | |
| 計算された分子量: | 86kDa/90kDa |
| 観察された分子量: | 110kDa |
| 同義語: | RASAL1, RASAL |
| バックグラウンド: | The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described |
| UniProt Protein Function: | RASAL1: a GTPase activating protein for Ras. Negatively regulates Ras (H-Ras) signaling activity. Two alternatively-spliced isoforms have been described. PITX1 inhibits tumorigenicity of the Ras pathway by inducing the transcription of RASAL1, which in turn inactivates Ras. Two alternatively-spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:GAPs, Ras; GAPs Chromosomal Location of Human Ortholog: 12q23-q24 Cellular Component: cytosol; plasma membrane Molecular Function:phospholipid binding Biological Process: MAPKKK cascade; negative regulation of Ras protein signal transduction; signal transduction |
| NCBI Summary: | The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described [provided by RefSeq, Jul 2010] |
| UniProt Code: | O95294 |
| NCBI GenInfo Identifier: | 311033542 |
| NCBI Gene ID: | 8437 |
| NCBI Accession: | O95294.3 |
| UniProt Secondary Accession: | O95294,Q52M03, Q59H24, Q96CC7, B7ZKM4, C9JFK5, F8VQX1 |
| UniProt Related Accession: | O95294 |
| Molecular Weight: | 90,145 Da |
| NCBI Full Name: | RasGAP-activating-like protein 1 |
| NCBI Synonym Full Names: | RAS protein activator like 1 |
| NCBI Official Symbol: | RASAL1 |
| NCBI Official Synonym Symbols: | RASAL |
| NCBI Protein Information: | rasGAP-activating-like protein 1 |
| UniProt Protein Name: | RasGAP-activating-like protein 1 |
| UniProt Synonym Protein Names: | RAS protein activator like 1 |
| Protein Family: | RasGAP-activating-like protein |
| UniProt Gene Name: | RASAL1 |
| UniProt Entry Name: | RASL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RASAL1 antibody (CAB15114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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