Anti-P2RX4 Antibody (CAB6682)
- SKU:
- CAB6682
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| 抗体名: | Anti-P2RX4 Antibody |
| 抗体コード: | CAB6682 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 55-338 of human P2RX4 (NP_002551.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 22Rv1, Mouse kidney, Mouse lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 55-338 of human P2RX4 (NP_002551.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QETD SVVS SVTT KVKG VAVT NTSK LGFR IWDV ADYV IPAQ EENS LFVM TNVI LTMN QTQG LCPE IPDA TTVC KSDA SCTA GSAG THSN GVST GRCV AFNG SVKT CEVA AWCP VEDD THVP QPAF LKAA ENFT LLVK NNIW YPKF NFSK RNIL PNIT TTYL KSCI YDAK TDPF CPIF RLGK IVEN AGHS FQDM AVEG GIMG IQVN WDCN LDRA ASLC LPRY SFRR LDTR DVEH NVSP GYNF RFAK YYRD LAGN EQRT LIKA YGIR FDII VFGK AGKF DIIP TMIN |
| 遺伝子ID: | 5025 |
| Uniprot: | Q99571 |
| セルラーロケーション: | Membrane, Multi-pass membrane protein |
| 計算された分子量: | 40kDa/43kDa/45kDa |
| 観察された分子量: | 62kDa |
| 同義語: | P2RX4, P2X4, P2X4R |
| バックグラウンド: | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene. |
| UniProt Protein Function: | P2X4: Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. Belongs to the P2X receptor family. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral Chromosomal Location of Human Ortholog: 12q24.32 Cellular Component: membrane; cell soma; perinuclear region of cytoplasm; apical part of cell; lysosomal membrane; integral to plasma membrane; postsynaptic density; dendritic spine; plasma membrane; terminal button; integral to nuclear inner membrane; cell junction Molecular Function:protein homodimerization activity; copper ion binding; zinc ion binding; cadherin binding; drug binding; ATP binding; purinergic nucleotide receptor activity; ATP-gated cation channel activity; receptor binding Biological Process: positive regulation of nitric oxide biosynthetic process; generation of action potential; positive regulation of calcium-mediated signaling; regulation of sodium ion transport; sensory perception of pain; signal transduction; vasodilation; response to ATP; endothelial cell activation; membrane depolarization; transport; calcium ion transport; regulation of blood pressure; positive regulation of prostaglandin secretion; tissue homeostasis; regulation of excitatory postsynaptic membrane potential; positive regulation of calcium ion transport; nitric oxide biosynthetic process; protein homooligomerization |
| NCBI Summary: | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q99571 |
| NCBI GenInfo Identifier: | 116242696 |
| NCBI Gene ID: | 5025 |
| NCBI Accession: | Q99571.2 |
| UniProt Related Accession: | Q99571 |
| Molecular Weight: | Predicted Molecular Mass: 33.2kDaAccurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions. |
| NCBI Full Name: | P2X purinoceptor 4 |
| NCBI Synonym Full Names: | purinergic receptor P2X 4 |
| NCBI Official Symbol: | P2RX4 |
| NCBI Official Synonym Symbols: | P2X4; P2X4R |
| NCBI Protein Information: | P2X purinoceptor 4 |
| UniProt Protein Name: | P2X purinoceptor 4 |
| UniProt Synonym Protein Names: | ATP receptor; Purinergic receptor |
| Protein Family: | P2X purinoceptor |
| UniProt Gene Name: | P2RX4 |
| UniProt Entry Name: | P2RX4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using P2RX4 antibody (CAB6682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of MCF7 cells using P2RX4 antibody (CAB6682). Blue: DAPI for nuclear staining. |