Anti-NR3C1 Antibody (RACO0302)
- SKU:
- RACO0302
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | NR3C1 Antibody |
| Product SKU: | RACO0302 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, FC |
| 推奨される希釈: | WB:1:500-1:5000, FC:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human GR |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Receptor for glucocorticoids (GC) (PubMed:27120390). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). |
| シノニム: | Glucocorticoid receptor (GR) (Nuclear receptor subfamily 3 group C member 1), NR3C1, GRL |
 | Overlay histogram showing Jurkat cells stained with RACO0302 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg) 1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg) 1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
 | Western Blot. Positive WB detected in 293 whole cell lysate Hela whole cell lysate HepG2 whole cell lysate Jurkat whole cell lysate A549 whole cell lysate MCF-7 whole cell lysate U87 whole cell lysate All lanes NR3C1 antibody at 1:1500. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 86, 83, 82, 77, 76, 65, 61, 52, 51, 50 kDa. Observed band size: 95 kDa. |
| UniProt Protein Function: | GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Mitochondrial; DNA-binding; Transcription factor; Nuclear receptor Chromosomal Location of Human Ortholog: 5q31.3 Cellular Component: cytoplasm; cytosol; membrane; mitochondrial matrix; nucleoplasm; nucleus Molecular Function:glucocorticoid receptor activity; protein binding; protein dimerization activity; steroid binding; transcription factor activity; zinc ion binding Biological Process: adrenal gland development; chromatin modification; gene expression; glucocorticoid mediated signaling; glucocorticoid metabolic process; glucocorticoid receptor signaling pathway; maternal behavior; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; regulation of glucocorticoid biosynthetic process; regulation of gluconeogenesis; regulation of transcription, DNA-dependent; signal transduction; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent Disease: Glucocorticoid Resistance, Generalized |
| NCBI Summary: | This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011] |
| UniProt Code: | P04150 |
| NCBI GenInfo Identifier: | 121069 |
| NCBI Gene ID: | 2908 |
| NCBI Accession: | P04150.1 |
| UniProt Secondary Accession: | P04150,P04151, Q53EP5, Q6N0A4, A0ZXF9, B0LPG8, D3DQF4 |
| UniProt Related Accession: | P04150 |
| Molecular Weight: | 82,904 Da |
| NCBI Full Name: | Glucocorticoid receptor |
| NCBI Synonym Full Names: | nuclear receptor subfamily 3 group C member 1 |
| NCBI Official Symbol: | NR3C1 |
| NCBI Official Synonym Symbols: | GR; GCR; GRL; GCCR; GCRST |
| NCBI Protein Information: | glucocorticoid receptor |
| UniProt Protein Name: | Glucocorticoid receptor |
| UniProt Synonym Protein Names: | Nuclear receptor subfamily 3 group C member 1 |
| Protein Family: | Glucocorticoid receptor |
| UniProt Gene Name: | NR3C1 |
| UniProt Entry Name: | GCR_HUMAN |