Anti-MRPS27 Antibody (CAB4527)
- SKU:
- CAB4527
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-MRPS27 Antibody |
| 抗体コード: | CAB4527 |
| 抗体サイズ: | 100µL |
| 申し込み: | WB IHC IF IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human MRPS27 |
| 申し込み: | WB IHC IF IP |
| 推奨希釈: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:20 - 1:50 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa |
| 免疫原: | Recombinant protein of human MRPS27 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 23107 |
| Uniprot: | Q92552 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 47kDa/49kDa |
| 観察された分子量: | 50kDa |
| 同義語: | MRPS27, MRP-S27, S27mt |
| バックグラウンド: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | MRPS27: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. |
| UniProt Protein Details: | Protein type:Ribosomal; Mitochondrial; Translation Chromosomal Location of Human Ortholog: 5q13.2 Cellular Component: mitochondrial inner membrane; ribosome Molecular Function:protein binding Biological Process: mitochondrial translation; organelle organization and biogenesis |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013] |
| UniProt Code: | Q92552 |
| NCBI GenInfo Identifier: | 209572725 |
| NCBI Gene ID: | 23107 |
| NCBI Accession: | Q92552.3 |
| UniProt Secondary Accession: | Q92552,Q6P1S1, B4DRT2, |
| UniProt Related Accession: | Q92552 |
| Molecular Weight: | 414 |
| NCBI Full Name: | 28S ribosomal protein S27, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein S27 |
| NCBI Official Symbol: | MRPS27 |
| NCBI Official Synonym Symbols: | S27mt; MRP-S27 |
| NCBI Protein Information: | 28S ribosomal protein S27, mitochondrial; mitochondrial 28S ribosomal protein S27 |
| UniProt Protein Name: | 28S ribosomal protein S27, mitochondrial |
| Protein Family: | 28S ribosomal protein |
| UniProt Gene Name: | MRPS27 |
| UniProt Entry Name: | RT27_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using MRPS27 antibody (CAB4527). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
View AllClose