Anti-MFI2 Antibody (CAB6653)
- SKU:
- CAB6653
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-MFI2 Antibody |
| 抗体コード: | CAB6653 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human MFI2 (NP_201573.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, SW480, A375, Mouse liver, Mouse brain, Mouse thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human MFI2 (NP_201573.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GMEV RWCA TSDP EQHK CGNM SEAF REAG IQPS LLCV RGTS ADHC VQLI AAQE ADAI TLDG GAIY EAGK EHGL KPVV GEVY DQEV GTSY YAVA VVRR SSHV TIDT LKGV KSCH TGIN RTVG WNVP VGYL VESG RLSV MGCD VLKA VSDY FGGS CVPG AGET SYSE SLCR LCRG DSSG EGVC D |
| 遺伝子ID: | 4241 |
| Uniprot: | P08582 |
| セルラーロケーション: | Cell membrane, GPI-anchor, Lipid-anchor |
| 計算された分子量: | 32kDa/80kDa |
| 観察された分子量: | 110kDa |
| 同義語: | MELTF, CD228, MAP97, MFI2, MTF1, MTf |
| バックグラウンド: | The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. |
| UniProt Protein Function: | MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor Chromosomal Location of Human Ortholog: 3q28-q29 Cellular Component: cell surface; anchored to plasma membrane; integral to plasma membrane Molecular Function:protein binding; ferric iron binding; iron ion binding Biological Process: cellular iron ion homeostasis; regulation of cell growth; regulation of cell proliferation |
| NCBI Summary: | The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P08582 |
| NCBI GenInfo Identifier: | 338817914 |
| NCBI Gene ID: | 4241 |
| NCBI Accession: | P08582.2 |
| UniProt Secondary Accession: | P08582,Q9BQE2, |
| UniProt Related Accession: | P08582 |
| Molecular Weight: | 738 |
| NCBI Full Name: | Melanotransferrin |
| NCBI Synonym Full Names: | antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 |
| NCBI Official Symbol: | MFI2 |
| NCBI Official Synonym Symbols: | MTF1; CD228; MAP97 |
| NCBI Protein Information: | melanotransferrin; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2 |
| UniProt Protein Name: | Melanotransferrin |
| UniProt Synonym Protein Names: | Melanoma-associated antigen p97; CD_antigen: CD228 |
| UniProt Gene Name: | MFI2 |
| UniProt Entry Name: | TRFM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MFI2 antibody (CAB6653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using MFI2 Rabbit pAb (CAB6653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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