Anti-MAML1 Antibody (CAB17060)
- SKU:
- CAB17060
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-MAML1 Antibody |
| 抗体コード: | CAB17060 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MAML1 (NP_055572.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, Jurkat, mouse brain, rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MAML1 (NP_055572.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MVLP TCPM AEFA LPRH SAVM ERLR RRIE LCRR HHST CEAR YEAV SPER LELE RQHT FALH QRCI QAKA KRAG KHRQ PPAA TAPA PAAP APRL DAAD GPEH GRPA THLH DTVK RNLD SATS PQNG DQQN GYGD LFPG HKKT RREA PLGV AISS NGLP PASP LGQS DKPS GADA LQSS GKHS LGLD SLNK KRLA DSSL HLNG GSNP SESF PLSL NKEL KQEP VEDL PCMI TGTV GSIS QSNL MPDL NLNE QEWK ELIE ELNR SVPD EDMK DLFN EDFE EKKD PESS GSAT QTPL AQDI NIKT |
| 遺伝子ID: | 9794 |
| Uniprot: | Q92585 |
| セルラーロケーション: | |
| 計算された分子量: | 108kDa |
| 観察された分子量: | 120kDa |
| 同義語: | MAML1, Mam-1, Mam1 |
| バックグラウンド: | This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. |
| UniProt Protein Function: | MAML1: Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. Interacts (via N-terminus) with NOTCH1, NOTCH2, NOTCH3 and NOTCH4 (via ankyrin repeat region). Interacts (via N-terminus) with p53 (via DNA-binding region). Forms a DNA-binding complex with Notch proteins and RBPSUH/RBP-J kappa/CBF1. Also binds CREBBP/CBP and CDK8. Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. Belongs to the mastermind family. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 5q35 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus Molecular Function:peptide antigen binding; protein binding; protein kinase binding; transcription coactivator activity Biological Process: activation of Notch receptor target transcription factor; Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q92585 |
| NCBI GenInfo Identifier: | 68565602 |
| NCBI Gene ID: | 9794 |
| NCBI Accession: | Q92585.3 |
| UniProt Secondary Accession: | Q92585,Q9NZ12, |
| UniProt Related Accession: | Q92585 |
| Molecular Weight: | 108,054 Da |
| NCBI Full Name: | Mastermind-like protein 1 |
| NCBI Synonym Full Names: | mastermind like transcriptional coactivator 1 |
| NCBI Official Symbol: | MAML1 |
| NCBI Official Synonym Symbols: | Mam1; Mam-1 |
| NCBI Protein Information: | mastermind-like protein 1 |
| UniProt Protein Name: | Mastermind-like protein 1 |
| UniProt Gene Name: | MAML1 |
| UniProt Entry Name: | MAML1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAML1 antibody (CAB17060) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
View AllClose