Anti-GIT1 Antibody (CAB15437)[KO Validated]
![Anti-GIT1 Antibody (CAB15437)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/22619/22467/anti-git1-antibody-cab15437ko-validated__85332__25448.1645805191.jpg?c=1 "Anti-GIT1 Antibody (CAB15437)[KO Validated]")
- SKU:
- CAB15437
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
![Anti-GIT1 Antibody (CAB15437)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/22619/22467/anti-git1-antibody-cab15437ko-validated__85332__25448.1645805191.jpg?c=1&imbypass=on "Anti-GIT1 Antibody (CAB15437)[KO Validated]")
Frequently bought together:
Description
| 抗体名: | Anti-GIT1 Antibody [KO Validated] |
| 抗体コード: | CAB15437 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-251MG, A-549, A375, Mouse heart, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EIHK LQAE NLQL RQPP GPVP TPPL PSER AEHT PMAP GGST HRRD RQAF SMYE PGSA LKPF GGPP GDEL TTRL QPFH STEL EDDA IYSV HVPA GLYR IRKG VSAS AVPF TPSS PLLS CSQE GSRH TSKL SRHG SGAD SDYE NTQS GDPL LGLE GKRF LELG KEED FHPE LESL DGDL DPGL P |
| 遺伝子ID: | 28964 |
| Uniprot: | Q9Y2X7 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 19kDa/84kDa/85kDa |
| 観察された分子量: | 100kDa |
| 同義語: | GIT1 |
| バックグラウンド: |
| UniProt Protein Function: | GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs). |
| UniProt Protein Details: | Protein type:GAPs, ARF; Motility/polarity/chemotaxis; GAPs Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane Molecular Function:protein binding Biological Process: ephrin receptor signaling pathway; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway |
| UniProt Code: | Q9Y2X7 |
| NCBI GenInfo Identifier: | 45645212 |
| NCBI Gene ID: | 28964 |
| NCBI Accession: | Q9Y2X7.2 |
| UniProt Secondary Accession: | Q9Y2X7,Q86SS0, Q9BRJ4, B4DGU9, B4DSV3, |
| UniProt Related Accession: | Q9Y2X7 |
| Molecular Weight: | 85,446 Da |
| NCBI Full Name: | ARF GTPase-activating protein GIT1 |
| NCBI Synonym Full Names: | GIT ArfGAP 1 |
| NCBI Official Symbol: | GIT1 |
| NCBI Protein Information: | ARF GTPase-activating protein GIT1 |
| UniProt Protein Name: | ARF GTPase-activating protein GIT1 |
| UniProt Synonym Protein Names: | Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1 |
| Protein Family: | ARF GTPase-activating protein |
| UniProt Gene Name: | GIT1 |
| UniProt Entry Name: | GIT1_HUMAN |
![Anti-GIT1 Antibody [KO Validated] (CAB15437)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/g/098/A15437_1__35842.jpg) | Western blot analysis of extracts of various cell lines, using GIT1 antibody (CAB15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![Anti-GIT1 Antibody [KO Validated] (CAB15437)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/r/420/A15437_2__53945.jpg) | Western blot analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using GIT1 antibody (CAB15437) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![Anti-GIT1 Antibody [KO Validated] (CAB15437)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/c/601/A15437_3__31922.jpg) | Immunofluorescence analysis of C6 cells using [KO] GIT1 antibody (CAB15437) at dilution of 1:100. Blue: DAPI for nuclear staining. |
![Anti-GIT1 Antibody [KO Validated] (CAB15437)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/v/384/A15437_4__22361.jpg) | Immunofluorescence analysis of L929 cells using [KO] GIT1 antibody (CAB15437) at dilution of 1:100. Blue: DAPI for nuclear staining. |
![Anti-GIT1 Antibody [KO Validated] (CAB15437)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/x/053/A15437_5__60677.jpg) | Immunofluorescence analysis of U-2 OS cells using [KO] GIT1 antibody (CAB15437) at dilution of 1:100. Blue: DAPI for nuclear staining. |
View AllClose