Anti-FXYD1 Antibody (CAB15082)
- SKU:
- CAB15082
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-FXYD1 Antibody |
| 抗体コード: | CAB15082 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-92 of human FXYD1 (NP_005022.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | mouse brain, mouse heart, mouse liver, rat heart, rat brain |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-92 of human FXYD1 (NP_005022.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MASL GHIL VFCV GLLT MAKA ESPK EHDP FTYD YQSL QIGG LVIA GILF ILGI LIVL SRRC RCKF NQQQ RTGE PDEE EGTF RSSI RRLS TRRR |
| 遺伝子ID: | 5348 |
| Uniprot: | O00168 |
| セルラーロケーション: | Membrane, Single-pass type I membrane protein |
| 計算された分子量: | 10kDa |
| 観察された分子量: | 16kDa |
| 同義語: | FXYD1, PLM |
| バックグラウンド: | This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na, K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Transcript variants with different 5' UTR sequences have been described in the literature. |
| UniProt Protein Function: | PLM: a protein of the FXYD family of small ion transport regulators or channels. Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. May have a functional role in muscle contraction. Major plasma membrane substrate for PKA and PKC in several different tissues. Phosphorylated in response to insulin and adrenergic stimulation.Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. |
| UniProt Protein Details: | Protein type:Channel, misc.; Cell surface; Membrane protein, integral Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: integral to plasma membrane; plasma membrane; sarcolemma; sodium:potassium-exchanging ATPase complex Molecular Function:sodium channel regulator activity Biological Process: chloride transport; muscle contraction; regulation of heart contraction |
| NCBI Summary: | This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Transcript variants with different 5' UTR sequences have been described in the literature. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00168 |
| NCBI GenInfo Identifier: | 22654269 |
| NCBI Gene ID: | 5348 |
| NCBI Accession: | O00168.2 |
| UniProt Secondary Accession: | O00168,A8K196, |
| UniProt Related Accession: | O00168 |
| Molecular Weight: | 10,441 Da |
| NCBI Full Name: | Phospholemman |
| NCBI Synonym Full Names: | FXYD domain containing ion transport regulator 1 |
| NCBI Official Symbol: | FXYD1 |
| NCBI Official Synonym Symbols: | PLM |
| NCBI Protein Information: | phospholemman |
| UniProt Protein Name: | Phospholemman |
| UniProt Synonym Protein Names: | FXYD domain-containing ion transport regulator 1 |
| Protein Family: | Phospholemman |
| UniProt Gene Name: | FXYD1 |
| UniProt Entry Name: | PLM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FXYD1 antibody (CAB15082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90. |
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