Anti-FBXO11 Antibody (CAB6153)
- SKU:
- CAB6153
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-FBXO11 Antibody |
| 抗体コード: | CAB6153 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 688-927 of human FBXO11 (NP_001177203.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:20 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HeLa, Mouse spleen, Mouse ovary, Mouse lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 688-927 of human FBXO11 (NP_001177203.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GILV YNSG LGCI EDNE IFDN AMAG VWIK TDSN PTLR RNKI HDGR DGGI CIFN GGRG LLEE NDIF RNAQ AGVL ISTN SHPI LRKN RIFD GFAA GIEI TNHA TATL EGNQ IFNN RFGG LFLA SGVN VTMK DNKI MNNQ DAIE KAVS RGQC LYKI SSYT SYPM HDFY RCHT CNTT DRNA ICVN CIKK CHQG HDVE FIRH DRFF CDCG AGTL SNPC TLAG EPTH DTDT LYDS APPI ESNT LQHN |
| 遺伝子ID: | 80204 |
| Uniprot: | Q86XK2 |
| セルラーロケーション: | Chromosome, Nucleus |
| 計算された分子量: | 23kDa/62kDa/65kDa/94kDa/103kDa/106kDa |
| 観察された分子量: | 130kDa |
| 同義語: | FBXO11, FBX11, PRMT9, UBR6, UG063H01, VIT1 |
| バックグラウンド: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. |
| UniProt Protein Function: | PRMT9: Substrate recognition component of the a (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. SCF(FBXO11) does not seem to direct ubiquitination of TP53. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 2p16.3 Cellular Component: cytoplasm; nucleolus; chromosome; nucleus; ubiquitin ligase complex Molecular Function:protein binding; protein-arginine N-methyltransferase activity; zinc ion binding; ubiquitin-protein ligase activity Biological Process: ubiquitin-dependent protein catabolic process; sensory perception of sound; protein ubiquitination; protein modification process; peptidyl-arginine N-methylation |
| NCBI Summary: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2010] |
| UniProt Code: | Q86XK2 |
| NCBI GenInfo Identifier: | 124012093 |
| NCBI Gene ID: | 80204 |
| NCBI Accession: | Q86XK2.3 |
| UniProt Secondary Accession: | Q86XK2,Q52ZP1, Q53EP7, Q53RT5, Q8IXG3, Q96E90, Q9H6V8 Q9H9L1, Q9NR14, Q9UFK1, Q9UHI1, A1L491, |
| UniProt Related Accession: | Q86XK2 |
| Molecular Weight: | 927 |
| NCBI Full Name: | F-box only protein 11 |
| NCBI Synonym Full Names: | F-box protein 11 |
| NCBI Official Symbol: | FBXO11 |
| NCBI Official Synonym Symbols: | UBR6; VIT1; FBX11; PRMT9; UG063H01 |
| NCBI Protein Information: | F-box only protein 11; vitiligo-associated protein 1; vitiligo-associated protein VIT-1; protein arginine N-methyltransferase 9; ubiquitin protein ligase E3 component n-recognin 6 |
| UniProt Protein Name: | F-box only protein 11 |
| UniProt Synonym Protein Names: | Protein arginine N-methyltransferase 9; Vitiligo-associated protein 1; VIT-1 |
| Protein Family: | F-box only protein |
| UniProt Gene Name: | FBXO11 |
| UniProt Entry Name: | FBX11_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FBXO11 antibody (CAB6153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of MCF-7 cells using FBXO11 antibody (CAB6153). Blue: DAPI for nuclear staining. |
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