Anti-CLIC4 Antibody (CAB7088)
- SKU:
- CAB7088
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-CLIC4 Antibody |
| 抗体コード: | CAB7088 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human CLIC4 (NP_039234.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SW480, HeLa, Jurkat, Raji, Mouse kidney, Mouse lung, Mouse small intestine, Mouse esophagus, Rat kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human CLIC4 (NP_039234.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MALS MPLN GLKE EDKE PLIE LFVK AGSD GESI GNCP FSQR LFMI LWLK GVVF SVTT VDLK RKPA DLQN LAPG THPP FITF NSEV KTDV NKIE EFLE EVLC PPKY LKLS PKHP ESNT AGMD IFAK FSAY IKNS RPEA NEAL ERGL LKTL QKLD EYLN SPLP DEID ENSM EDIK FSTR KFLD GNEM TLAD CNLL PKLH IVKV VAKK YRNF DIPK EMTG IWRY LTNA YSRD EFTN TCPS DKEV EIAY SDVA KRLT K |
| 遺伝子ID: | 25932 |
| Uniprot: | Q9Y696 |
| セルラーロケーション: | Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle membrane, Mitochondrion, Nucleus matrix, Single-pass membrane protein, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 28kDa |
| 観察された分子量: | 29kDa |
| 同義語: | CLIC4, CLIC4L, H1, MTCLIC, huH1, p64H1 |
| バックグラウンド: | Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). |
| UniProt Protein Function: | CLIC4: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell- surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical- basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Belongs to the chloride channel CLIC family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 1p36.11 Cellular Component: centrosome; cell surface; nuclear matrix; microvillus; cytoplasmic vesicle membrane; mitochondrion; intercellular junction; cytosol; actin cytoskeleton; microtubule cytoskeleton; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; intracellular; midbody Molecular Function:chloride channel activity; protein binding; voltage-gated ion channel activity Biological Process: keratinocyte differentiation; vacuolar acidification; fertilization; branching morphogenesis of a tube; apoptosis; multicellular organism growth; establishment and/or maintenance of apical/basal cell polarity; chloride transport; angiogenesis; endothelial cell morphogenesis; cell differentiation; negative regulation of cell migration; regulation of cytoskeleton organization and biogenesis |
| NCBI Summary: | Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y696 |
| NCBI GenInfo Identifier: | 20141285 |
| NCBI Gene ID: | 25932 |
| NCBI Accession: | Q9Y696.4 |
| UniProt Secondary Accession: | Q9Y696,Q9UFW9, Q9UQJ6, |
| UniProt Related Accession: | Q9Y696 |
| Molecular Weight: | 253 |
| NCBI Full Name: | Chloride intracellular channel protein 4 |
| NCBI Synonym Full Names: | chloride intracellular channel 4 |
| NCBI Official Symbol: | CLIC4 |
| NCBI Official Synonym Symbols: | H1; huH1; p64H1; CLIC4L; MTCLIC |
| NCBI Protein Information: | chloride intracellular channel protein 4; chloride intracellular channel 4 like; intracellular chloride ion channel protein p64H1 |
| UniProt Protein Name: | Chloride intracellular channel protein 4 |
| UniProt Synonym Protein Names: | Intracellular chloride ion channel protein p64H1 |
| Protein Family: | Chloride intracellular channel protein |
| UniProt Gene Name: | CLIC4 |
| UniProt Entry Name: | CLIC4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CLIC4 antibody (CAB7088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2s. |
 | Immunohistochemistry of paraffin-embedded rat brain using CLIC4 antibody (CAB7088) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A-549 cells using CLIC4 antibody (CAB7088). Blue: DAPI for nuclear staining. |
View AllClose