Anti-CBLB Antibody (CAB2014)
- SKU:
- CAB2014
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-CBLB Antibody |
| 抗体コード: | CAB2014 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CBLB (NP_733762.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Jurkat, 293T, Mouse spleen |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CBLB (NP_733762.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MANS MNGR NPGG RGGN PRKG RILG IIDA IQDA VGPP KQAA ADRR TVEK TWKL MDKV VRLC QNPK LQLK NSPP YILD ILPD TYQH LRLI LSKY DDNQ KLAQ LSEN EYFK IYID SLMK KSKR AIRL FKEG KERM YEEQ SQDR RNLT KLSL IFSH MLAE IKAI FPNG QFQG DNFR ITKA DAAE FWRK FFGD KTIV PWKV FRQC LHEV HQIS SGLE AMAL KSTI DLTC NDYI SVFE FDIF TRLF QPWG SILR NWNF LAVT HPGY MAFL TYDE VKAR LQKY STKP GSYI FRLS CTRL GQWA IGYV TGDG NILQ TIPH NKPL FQAL IDGS REGF YLYP DGRS YNPD LTGL CEPT PH |
| 遺伝子ID: | 868 |
| Uniprot: | Q13191 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 86kDa/90kDa/109kDa |
| 観察された分子量: | 109kDa |
| 同義語: | CBLB, Cbl-b, Nbla00127, RNF56 |
| バックグラウンド: |
| UniProt Protein Function: | Cbl-b: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B- cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T- cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization. Interacts with SH3 domain-containing proteins LCK, CRK and SORBS1. Interacts with LCP2 and ZAP70. May interact with CBL. Interacts with SH3 domain-containing proteins VAV1, FYN, FGR, PLCG1, GRB2, CRKL, PIK3R1 and SH3KBP1/CIN85. Identified in heterotrimeric complexes with SH3KBP1/CIN85, CD2AP and ARHGEF7, where one CBLB peptide binds two copies of the other protein. Interacts with poly-ubiquitinated proteins. Dimerization is required for the binding of poly-ubiquitin, but not for the binding of mono-ubiquitin. Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Calcium-binding; Ubiquitin ligase; Adaptor/scaffold; EC 6.3.2.-; Ubiquitin conjugating system; Ligase Chromosomal Location of Human Ortholog: 3q13.11 Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol Molecular Function:signal transducer activity; protein binding; zinc ion binding; phosphotyrosine binding; ubiquitin-protein ligase activity; calcium ion binding; ligase activity Biological Process: cell surface receptor linked signal transduction; T cell activation; positive regulation of protein catabolic process; negative regulation of alpha-beta T cell proliferation; negative regulation of T cell receptor signaling pathway; protein ubiquitination; immune response; NLS-bearing substrate import into nucleus; signal transduction; positive regulation of T cell anergy |
| UniProt Code: | Q13191 |
| NCBI GenInfo Identifier: | 88911265 |
| NCBI Gene ID: | 868 |
| NCBI Accession: | Q13191.2 |
| UniProt Secondary Accession: | Q13191,Q13192, Q13193, Q3LIC0, Q63Z43, Q8IVC5, A8K9S7 B7WNM4, |
| UniProt Related Accession: | Q13191 |
| Molecular Weight: | Calculated MW: 86kDa/90kDa/109kDaObserved MW: 109kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase CBL-B |
| NCBI Synonym Full Names: | Cbl proto-oncogene B, E3 ubiquitin protein ligase |
| NCBI Official Symbol: | CBLB |
| NCBI Official Synonym Symbols: | Cbl-b; RNF56; Nbla00127 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase CBL-B; RING finger protein 56; SH3-binding protein CBL-B; signal transduction protein CBL-B; casitas B-lineage lymphoma proto-oncogene b; Cbl proto-oncogene, E3 ubiquitin protein ligase B; Cas-Br-M (murine) ectropic retroviral transforming sequence b; Cas-Br-M (murine) ecotropic retroviral transforming sequence b |
| UniProt Protein Name: | E3 ubiquitin-protein ligase CBL-B |
| UniProt Synonym Protein Names: | Casitas B-lineage lymphoma proto-oncogene b; RING finger protein 56; SH3-binding protein CBL-B; Signal transduction protein CBL-B |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | CBLB |
| UniProt Entry Name: | CBLB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CBLB antibody (CAB2014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human liver damage using CBLB antibody (CAB2014) at dilution of 1:200 (40x lens). |
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