Anti-CAT Antibody (CAB11777)
- SKU:
- CAB11777
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| 抗体名: | Anti-CAT Antibody |
| 抗体コード: | CAB11777 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human CAT (NP_001743.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, U-87MG, Mouse kidney, Mouse liver, Mouse brain, Rat kidney, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human CAT (NP_001743.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MADS RDPA SDQM QHWK EQRA AQKA DVLT TGAG NPVG DKLN VITV GPRG PLLV QDVV FTDE MAHF DRER IPER VVHA KGAG AFGY FEVT HDIT KYSK AKVF EHIG KKTP IAVR FSTV AGES GSAD TVRD PRGF AVKF YTED GNWD LVGN NTPI FFIR DPIL FPSF IHSQ KRNP QTHL KDPD MVWD FWSL RPES LHQV SFLF SDRG IPDG HRHM NGYG SHTF KLVN A |
| 遺伝子ID: | 847 |
| Uniprot: | P04040 |
| セルラーロケーション: | Peroxisome |
| 計算された分子量: | 59kDa |
| 観察された分子量: | 60kDa |
| 同義語: | CAT, catalase, MGC138422, MGC138424 |
| バックグラウンド: | This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. |
| UniProt Protein Function: | Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - tryptophan; Endoplasmic reticulum; EC 1.11.1.6; Apoptosis; Energy Metabolism - methane; Mitochondrial; Hydrolase; Oxidoreductase Chromosomal Location of Human Ortholog: 11p13 Cellular Component: peroxisomal membrane; Golgi apparatus; peroxisomal matrix; focal adhesion; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; lysosome; plasma membrane; mitochondrial intermembrane space; peroxisome; cytosol Molecular Function:antioxidant activity; oxidoreductase activity, acting on peroxide as acceptor; enzyme binding; protein homodimerization activity; catalase activity; metal ion binding; heme binding; NADP binding; aminoacylase activity; receptor binding Biological Process: cholesterol metabolic process; hemoglobin metabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; activation of NF-kappaB transcription factor; protein homotetramerization; osteoblast differentiation; positive regulation of phosphoinositide 3-kinase cascade; response to vitamin E; response to reactive oxygen species; triacylglycerol metabolic process; response to hyperoxia; UV protection; hydrogen peroxide catabolic process; inhibition of NF-kappaB transcription factor; ureteric bud development; positive regulation of cell division; response to hypoxia; aerobic respiration; purine nucleotide catabolic process; protein tetramerization; negative regulation of apoptosis; aging Disease: Acatalasemia |
| NCBI Summary: | This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009] |
| UniProt Code: | P04040 |
| NCBI GenInfo Identifier: | 115702 |
| NCBI Gene ID: | 847 |
| NCBI Accession: | P04040.3 |
| UniProt Related Accession: | P04040 |
| Molecular Weight: | |
| NCBI Full Name: | Catalase |
| NCBI Synonym Full Names: | catalase |
| NCBI Official Symbol: | CAT |
| NCBI Protein Information: | catalase |
| UniProt Protein Name: | Catalase |
| Protein Family: | Catalase |
| UniProt Gene Name: | CAT |
| UniProt Entry Name: | CATA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CAT Antibody (CAB11777) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of HeLa cells using CAT antibody (CAB11777) at dilution of 1:100. Blue: DAPI for nuclear staining. |