Anti-ABCB11 Antibody (CAB8467)
- SKU:
- CAB8467
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-ABCB11 Antibody |
| 抗体コード: | CAB8467 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 600-750 of human ABCB11 (NP_003733.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | LO2, Mouse pancreas |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 600-750 of human ABCB11 (NP_003733.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VLSK IQHG HTII SVAH RLST VRAA DTII GFEH GTAV ERGT HEEL LERK GVYF TLVT LQSQ GNQA LNEE DIKD ATED DMLA RTFS RGSY QDSL RASI RQRS KSQL SYLV HEPP LAVV DHKS TYEE DRKD KDIP VQEE VEPA PVRR ILKF SAP |
| 遺伝子ID: | 8647 |
| Uniprot: | O95342 |
| セルラーロケーション: | Membrane, Multi-pass membrane protein |
| 計算された分子量: | 146kDa |
| 観察された分子量: | 170kDa |
| 同義語: | ABCB11, ABC16, BRIC2, BSEP, PFIC-2, PFIC2, PGY4, SPGP |
| バックグラウンド: | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. |
| UniProt Protein Function: | ABCB11: Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease. Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2). BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family; Transporter; Hydrolase Chromosomal Location of Human Ortholog: 2q24 Cellular Component: intercellular canaliculus; membrane; apical part of cell; integral to plasma membrane; plasma membrane; integral to membrane Molecular Function:transporter activity; ATPase activity, coupled to transmembrane movement of substances; canalicular bile acid transmembrane transporter activity; bile acid-exporting ATPase activity; sodium-exporting ATPase activity, phosphorylative mechanism; ATP binding Biological Process: bile acid and bile salt transport; bile acid biosynthetic process; bile acid metabolic process; transport; canalicular bile acid transport; transmembrane transport Disease: Cholestasis, Progressive Familial Intrahepatic, 2; Cholestasis, Benign Recurrent Intrahepatic, 2 |
| NCBI Summary: | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95342 |
| NCBI GenInfo Identifier: | 262527527 |
| NCBI Gene ID: | 8647 |
| NCBI Accession: | O95342.2 |
| UniProt Related Accession: | O95342 |
| Molecular Weight: | |
| NCBI Full Name: | Bile salt export pump |
| NCBI Synonym Full Names: | ATP binding cassette subfamily B member 11 |
| NCBI Official Symbol: | ABCB11 |
| NCBI Official Synonym Symbols: | BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2 |
| NCBI Protein Information: | bile salt export pump |
| UniProt Protein Name: | Bile salt export pump |
| UniProt Synonym Protein Names: | ATP-binding cassette sub-family B member 11 |
| UniProt Gene Name: | ABCB11 |
| UniProt Entry Name: | ABCBB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ABCB11 antibody (CAB8467) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
 | Immunofluorescence analysis of NIH-3T3 cells using Anti-ABCB11 Polyclonal Antibody (CAB8467) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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