Rat Dihydrolipoyl dehydrogenase, mitochondrial / DLD ELISA Kit

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SKU:
RTFI00407
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6P6R2
Sensitivity:
37.5pg/ml
Range:
62.5-4000pg/ml
ELISA Type:
Sandwich ELISA, Double Antibody
Synonyms:
Dld, Diaphorase, DLD, DLDD, DLDH, GCSL, LAD, Lipoamide Dehydrogenase, PHE3, 2-oxo-glutaRate complex, branched chain keto acid dehydrogenase complex, branched chain keto acid dehydrogenase complex, dihydrolipoamide dehydrogenase, E3 component of pyruv
Reactivity:
Rat
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Description

system_update_altデータシートsystem_update_alt安全性データシート
商品名:Rat Dld (Dihydrolipoyl dehydrogenase, mitochondrial) ELISA Kit
製品コード:RTFI00407
サイズ:96 Assays
目標:Rat Dld
エイリアス:Dld, Diaphorase, DLD, DLDD, DLDH, GCSL, LAD, Lipoamide Dehydrogenase, PHE3, 2-oxo-glutaRate complex, branched chain keto acid dehydrogenase complex
反応性:Rat
検出方法:Sandwich ELISA, Double Antibody
感度:37.5pg/ml
範囲:62.5-4000pg/ml
保管所:4°C for 6 months
ノート:For Research Use Only
回復:Matrices listed below were spiked with certain level of Rat Dld and the recovery rates were calculated by comparing the measured value to the expected amount of Rat Dld in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)88-9994
EDTA plasma(n=5)89-10096
UFH plasma(n=5)85-10092
直線性:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat Dld and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)86-100%90-105%86-105%
EDTA plasma(n=5)83-92%92-99%88-101%
UFH plasma(n=5)81-100%80-88%83-89%
Intra-Assay:CV <8%
Inter-Assay:CV <10%
Uniprot:Q6P6R2
UniProt Protein Function:DLD: a multi-functional mitochondrial enzyme. An enzymatic component of the mitochondrial glycine cleavage system, the pyruvate dehydrogenase complex (PDHC), the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Is the E3 component of the PDHC that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. The E3 component has dihydrolipoamide dehydrogenase activity. The PDHC contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. Defects in DLD are a cause of maple syrup urine disease (MSUD), characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation. Differentially expressed in the Wernicke's Area from patients with schizophrenia. Inhibited by 5-methoxyindole-2-carboxylic acid (MICA).
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.8.1.4; Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; Mitochondrial

Cellular Component: acrosomal matrix; cilium; mitochondrial matrix; mitochondrion; myelin sheath; nucleoplasm; oxoglutarate dehydrogenase complex; pyruvate dehydrogenase complex

Molecular Function:dihydrolipoyl dehydrogenase activity; FAD binding; lipoamide binding; NAD binding

Biological Process: 2-oxoglutarate metabolic process; acetyl-CoA biosynthetic process from pyruvate; aging; cell redox homeostasis; dihydrolipoamide metabolic process; gastrulation; lipoate metabolic process; mitochondrial electron transport, NADH to ubiquinone; proteolysis; regulation of membrane potential; sperm capacitation

NCBI Summary:This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. A similar protein in human, mouse, and pig has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, the protein can function as a protease. [provided by RefSeq, Jan 2014]
UniProt Code:Q6P6R2
NCBI GenInfo Identifier:40786469
NCBI Gene ID:298942
NCBI Accession:NP_955417.1
UniProt Related Accession:Q6P6R2
Molecular Weight:
NCBI Full Name:dihydrolipoyl dehydrogenase, mitochondrial
NCBI Synonym Full Names:dihydrolipoamide dehydrogenase
NCBI Official Symbol:Dld  
NCBI Protein Information:dihydrolipoyl dehydrogenase, mitochondrial
UniProt Protein Name:Dihydrolipoyl dehydrogenase, mitochondrial
UniProt Synonym Protein Names:Dihydrolipoamide dehydrogenase
Protein Family:Delta-like protein
UniProt Gene Name:Dld  
UniProt Entry Name:DLDH_RAT
ステッププロトコル
1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3. Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4. Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37°C for 90 min.
6. Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7. Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9. Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10. Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11. Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12. Add 90 µL of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13. Add 50 µL of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

ELISAアッセイを実施する場合、可能な限り最良の結果を達成するためにサンプルを準備することが重要です。以下に、さまざまなサンプルタイプのサンプルを準備するための手順のリストを示します。

サンプルタイプ プロトコル
血清

If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clotovernight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Removeserum and assay promptly or aliquot and store the samples at-80°C. Avoid multiple freeze-thaw cycles.

プラズマ Collect plasma using EDTA or heparin as an anti-coagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.Note: Over haemolysed samples are not suitable for use with this kit.
尿および脳脊髄液 Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
細胞培養上清 Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
細胞溶解物 Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20°C.
組織ホモジネート The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenizein 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or-80°C.
組織溶解物 Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
母乳 Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.
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