Anti-ZFYVE27 Antibody (CAB15943)
- SKU:
- CAB15943
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ZFYVE27 Antibody |
| 抗体コード: | CAB15943 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 217-411 of human ZFYVE27 (NP_653189.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | DU145 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 217-411 of human ZFYVE27 (NP_653189.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VEFF RVVS EYRA SLQQ RMNP KQEE HAFE SPPP PDVG GKDG LMDS TPAL TPTE DLTP GSVE EAEE AEPD EEFK DAIE ETHL VVLE DDEG APCP AEDE LALQ DNGF LSKN EVLR SKVS RLTE RLRK RYPT NNFG NCTG CSAT FSVL KKRR SCSN CGNS FCSR CCSF KVPK SSMG ATAP EAQR ETVF VCAS CNQT LSK |
| 遺伝子ID: | 118813 |
| Uniprot: | Q5T4F4 |
| セルラーロケーション: | Cell membrane, Cell projection, Endoplasmic reticulum membrane, Multi-pass membrane protein, Recycling endosome membrane, growth cone membrane |
| 計算された分子量: | 31kDa/34kDa/35kDa/41kDa/45kDa/46kDa |
| 観察された分子量: | 46kDa |
| 同義語: | ZFYVE27, PROTRUDIN, SPG33, protrudin |
| バックグラウンド: | This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. |
| UniProt Protein Function: | ZFYVE27: Functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Involved in nerve growth factor-induced neurite formation. May have a more general role in cell projections formation. Defects in ZFYVE27 are the cause of spastic paraplegia autosomal dominant type 33 (SPG33). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. According to PubMed:18606302, the properties of the variant Val-191 and its frequency in some populations raise doubts on the implication of that gene in the disease. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 10q24.2 Cellular Component: axon; dendrite; endoplasmic reticulum; recycling endosome membrane Molecular Function:protein binding Biological Process: nerve growth factor receptor signaling pathway; neurite development Disease: Spastic Paraplegia 33, Autosomal Dominant |
| NCBI Summary: | This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010] |
| UniProt Code: | Q5T4F4 |
| NCBI GenInfo Identifier: | 74744927 |
| NCBI Gene ID: | 118813 |
| NCBI Accession: | Q5T4F4.1 |
| UniProt Secondary Accession: | Q5T4F4,Q5T4F1, Q5T4F2, Q5T4F3, Q8N1K0, Q8N6D6, B7Z3S0 B7Z404, B7Z626, G8JLC3, G8JLF0, J3KP98, |
| UniProt Related Accession: | Q5T4F4 |
| Molecular Weight: | 41,417 Da |
| NCBI Full Name: | Protrudin |
| NCBI Synonym Full Names: | zinc finger FYVE-type containing 27 |
| NCBI Official Symbol: | ZFYVE27 |
| NCBI Official Synonym Symbols: | SPG33; PROTRUDIN |
| NCBI Protein Information: | protrudin |
| UniProt Protein Name: | Protrudin |
| UniProt Synonym Protein Names: | Zinc finger FYVE domain-containing protein 27 |
| Protein Family: | Protrudin |
| UniProt Gene Name: | ZFYVE27 |
| UniProt Entry Name: | ZFY27_HUMAN |
 | Western blot analysis of extracts of DU145 cells, using ZFYVE27 antibody (CAB15943) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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