Anti-ZCCHC8 Antibody (CAB9563)
- SKU:
- CAB9563
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-ZCCHC8 Antibody |
| 抗体コード: | CAB9563 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZCCHC8 (NP_060082.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, Mouse thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZCCHC8 (NP_060082.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAAE VYFG DLEL FEPF DHPE ESIP KPVH TRFK DDDG DEED ENGV GDAE LRER LRQC EETI EQLR AENQ ELKR KLNI LTRP SGIL VNDT KLDG PILQ ILFM NNAI SKQY HQEI EEFV SNLV KRFE EQQK NDVE KTSF NLLP QPSS IVLE EDHK VEES CAIK NNKE AFSV VGSV LYFT NFCL DKLG QPLL NENP QLSE GWEI PKYH QVFS HIVS LEGQ EIQV KAKR PKPH CFNC GSEE HQMK |
| 遺伝子ID: | 55596 |
| Uniprot: | Q6NZY4 |
| セルラーロケーション: | Nucleus, nucleoplasm |
| 計算された分子量: | 51kDa/78kDa |
| 観察された分子量: | 78kDa |
| 同義語: | ZCCHC8 |
| バックグラウンド: | This gene encodes a scaffold protein which serves as an assessory factor to the nuclear RNA exosome complex. The encoded protein forms a trimeric human nuclear exosome targeting (NEXT) complex, together with hMTR4 and the RNA-binding factor RBM7 which promotes the exosomal degradation of non-coding promoter-upstream transcripts, enhancer RNAs and 3'-extended products of histone- and small nuclear RNA transcription. This complex is also thought to recruit the exosome to degrade intronic RNAs via its interaction with both the exosome and the spliceosome. It contains both an N-terminal zinc-knuckle domain and a C-terminal proline-rich domain. |
| UniProt Protein Function: | ZCCHC8: May be involved in pre-mRNA splicing. Belongs to the ZCCHC8 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Spliceosome Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: nucleus Molecular Function:protein binding Biological Process: nuclear mRNA splicing, via spliceosome |
| UniProt Code: | Q6NZY4 |
| NCBI GenInfo Identifier: | 66774213 |
| NCBI Gene ID: | 55596 |
| NCBI Accession: | Q6NZY4.2 |
| UniProt Secondary Accession: | Q6NZY4,Q7L2P6, Q8N2K5, Q96SK7, Q9NSS2, Q9NSS3, |
| UniProt Related Accession: | Q6NZY4 |
| Molecular Weight: | 51,130 Da |
| NCBI Full Name: | Zinc finger CCHC domain-containing protein 8 |
| NCBI Synonym Full Names: | zinc finger CCHC-type containing 8 |
| NCBI Official Symbol: | ZCCHC8 |
| NCBI Protein Information: | zinc finger CCHC domain-containing protein 8 |
| UniProt Protein Name: | Zinc finger CCHC domain-containing protein 8 |
| UniProt Synonym Protein Names: | TRAMP-like complex RNA-binding factor ZCCHC8 |
| Protein Family: | Zinc finger CCHC domain-containing protein |
| UniProt Gene Name: | ZCCHC8 |
| UniProt Entry Name: | ZCHC8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ZCCHC8 antibody (CAB9563) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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