Anti-ZAK Antibody (CAB7371)
- SKU:
- CAB7371
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Frequently bought together:
Description
| 抗体名: | Anti-ZAK Antibody |
| 抗体コード: | CAB7371 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ZAK (NP_598407.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, A-549, HepG2, Mouse lung, Mouse heart, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ZAK (NP_598407.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSSL GASF VQIK FDDL QFFE NCGG GSFG SVYR AKWI SQDK EVAV KKLL KIEK EAEI LSVL SHRN IIQF YGVI LEPP NYGI VTEY ASLG SLYD YINS NRSE EMDM DHIM TWAT DVAK GMHY LHME APVK VIHR DLKS RNVV IAAD GVLK ICDF GASR FHNH TTHM SLVG TFPW MAPE VIQS LPVS ETCD TYSY GVVL WEML TREV PFKG LEGL QVAW LVVE KNER LTIP SSCP RSFA ELLH QCWE ADAK KRPS FKQI ISIL ESMS NDTS LPDK CNSF LHNK AEWR CEIE ATLE RLKK LERD LSFK EQEL KERE RRLK MWEQ KLTE QSNT PL |
| 遺伝子ID: | 51776 |
| Uniprot: | |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 35kDa/51kDa/91kDa |
| 観察された分子量: | 55kDa/91kDa/100kDa |
| 同義語: | MAP3K20, AZK, MLK7, MLT, MLTK, MLTKalpha, MLTKbeta, MRK, SFMMP, ZAK, mlklak, pk |
| バックグラウンド: | This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
 | Western blot analysis of extracts of various cell lines, using ZAK antibody (CAB7371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using ZAK antibody (CAB7371) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using ZAK antibody (CAB7371) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using ZAK antibody (CAB7371). Blue: DAPI for nuclear staining. |