Anti-XDH Antibody (RACO0507)
- SKU:
- RACO0507
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | XDH Antibody |
| Product SKU: | RACO0507 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Xanthine Oxidase |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). |
| シノニム: | Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase (XD) (EC 1.17.1.4), Xanthine oxidase (XO) (EC 1.17.3.2) (Xanthine oxidoreductase) (XOR)], XDH, XDHA |
 | IHC image of RACO0507 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in Hela whole cell lysate All lanes Xanthine Oxidase antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 147 kDa. Observed band size: 147 kDa. |
| UniProt Protein Function: | XDH: Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). Defects in XDH are the cause of xanthinuria type 1 (XU1). Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. Belongs to the xanthine dehydrogenase family. |
| UniProt Protein Details: | Protein type:EC 1.17.1.4; Secondary Metabolites Metabolism - caffeine; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - purine; EC 1.17.3.2; Oxidoreductase Chromosomal Location of Human Ortholog: 2p23.1 Cellular Component: cytosol; extracellular space Molecular Function:2 iron, 2 sulfur cluster binding; FAD binding; molybdopterin cofactor binding; oxidoreductase activity, acting on the aldehyde or oxo group of donors; protein homodimerization activity; xanthine dehydrogenase activity; xanthine oxidase activity Biological Process: caspase activation; negative regulation of endothelial cell differentiation; negative regulation of endothelial cell proliferation; negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase B signaling cascade; purine nucleotide catabolic process; xanthine catabolic process Disease: Xanthinuria, Type I |
| NCBI Summary: | Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014] |
| UniProt Code: | P47989 |
| NCBI GenInfo Identifier: | 2506326 |
| NCBI Gene ID: | 7498 |
| NCBI Accession: | P47989.4 |
| UniProt Secondary Accession: | P47989,Q16681, Q16712, Q4PJ16, |
| UniProt Related Accession: | P47989 |
| Molecular Weight: | 146,424 Da |
| NCBI Full Name: | Xanthine dehydrogenase/oxidase |
| NCBI Synonym Full Names: | xanthine dehydrogenase |
| NCBI Official Symbol: | XDH |
| NCBI Official Synonym Symbols: | XO; XOR |
| NCBI Protein Information: | xanthine dehydrogenase/oxidase |
| UniProt Protein Name: | Xanthine dehydrogenase/oxidase |
| UniProt Synonym Protein Names: | Xanthine oxidoreductase; XOR |
| Protein Family: | Xanthine dehydrogenase |
| UniProt Gene Name: | XDH |
| UniProt Entry Name: | XDH_HUMAN |