Anti-WDR4 Antibody (CAB16487)
- SKU:
- CAB16487
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-WDR4 Antibody |
| 抗体コード: | CAB16487 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-250 of human WDR4 (NP_061139.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | HeLa, HepG2, Rat lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-250 of human WDR4 (NP_061139.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | SRFL ATSI ASSD DDSL FIYD CSAA EKKS QENK GEDA PLDQ GSGA ILAS TFSK SGSY FALT DDSK RLIL FRTK PWQC LSVR TVAR RCTA LTFI ASEE KVLV ADKS GDVY SFSV LEPH GCGR LELG HLSM LLDV AVSP DDRF ILTA DRDE KIRV SWAA APHS IESF CLGH TEFV SRIS VVPT QPGL LLSS SGDG TLRL WEYR SGRQ LHCC HLAS LQEL VDPQ APQK FAAS RIA |
| 遺伝子ID: | 10785 |
| Uniprot: | P57081 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 29kDa/45kDa |
| 観察された分子量: | 45kDa |
| 同義語: | WDR4, TRM82, TRMT82 |
| バックグラウンド: | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | WDR4: a WD40 repeat protein. Required for 7-methylguanosine modification of tRNA. Forms a complex with METTL1. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding; tRNA (guanine-N7-)-methyltransferase activity Biological Process: tRNA modification |
| NCBI Summary: | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | P57081 |
| NCBI GenInfo Identifier: | 20141943 |
| NCBI Gene ID: | 10785 |
| NCBI Accession: | P57081.2 |
| UniProt Secondary Accession: | P57081,Q9BVM5, Q9HCR3, A8KA58, B2RCA3, B4DNQ7, D3DSK3 |
| UniProt Related Accession: | P57081 |
| Molecular Weight: | 29,946 Da |
| NCBI Full Name: | tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 |
| NCBI Synonym Full Names: | WD repeat domain 4 |
| NCBI Official Symbol: | WDR4 |
| NCBI Official Synonym Symbols: | TRM82; TRMT82 |
| NCBI Protein Information: | tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 |
| UniProt Protein Name: | tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 |
| UniProt Synonym Protein Names: | WD repeat-containing protein 4 |
| Protein Family: | tRNA |
| UniProt Gene Name: | WDR4 |
| UniProt Entry Name: | WDR4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WDR4 antibody (CAB16487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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