Anti-VASP Antibody (CAB14217)
- SKU:
- CAB14217
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-VASP Antibody |
| 抗体コード: | CAB14217 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VASP (NP_003361.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | A-431, Mouse kidney |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VASP (NP_003361.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSET VICS SRAT VMLY DDGN KRWL PAGT GPQA FSRV QIYH NPTA NSFR VVGR KMQP DQQV VINC AIVR GVKY NQAT PNFH QWRD ARQV WGLN FGSK EDAA |
| 遺伝子ID: | 7408 |
| Uniprot: | P50552 |
| セルラーロケーション: | Cell junction, Cell projection, Cytoplasm, cytoskeleton, filopodium membrane, focal adhesion, lamellipodium membrane, tight junction |
| 計算された分子量: | 39kDa |
| 観察された分子量: | 45kDa |
| 同義語: | VASP |
| バックグラウンド: | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. |
| UniProt Protein Function: | VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 19q13.32 Cellular Component: filopodium membrane; tight junction; focal adhesion; cytoplasm; plasma membrane; cytosol; actin cytoskeleton Molecular Function:protein binding; actin binding; SH3 domain binding; profilin binding Biological Process: axon guidance; positive regulation of actin filament polymerization; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway; protein homotetramerization |
| NCBI Summary: | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50552 |
| NCBI GenInfo Identifier: | 1718079 |
| NCBI Gene ID: | 7408 |
| NCBI Accession: | P50552.3 |
| UniProt Secondary Accession: | P50552,Q6PIZ1, Q93035, B2RBT9, |
| UniProt Related Accession: | P50552 |
| Molecular Weight: | 380 |
| NCBI Full Name: | Vasodilator-stimulated phosphoprotein |
| NCBI Synonym Full Names: | vasodilator-stimulated phosphoprotein |
| NCBI Official Symbol: | VASP |
| NCBI Protein Information: | vasodilator-stimulated phosphoprotein |
| UniProt Protein Name: | Vasodilator-stimulated phosphoprotein |
| Protein Family: | Vasodilator-stimulated phosphoprotein |
| UniProt Gene Name: | VASP |
| UniProt Entry Name: | VASP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VASP antibody (CAB14217) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using VASP antibody (CAB14217) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using VASP antibody (CAB14217) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using VASP antibody (CAB14217) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using VASP Rabbit pAb (CAB14217) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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