Anti-ULK3 Antibody (CAB7587)
- SKU:
- CAB7587
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| 抗体名: | Anti-ULK3 Antibody |
| 抗体コード: | CAB7587 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 44-240 of human ULK3 (NP_001092906.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | A-549, HeLa, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 44-240 of human ULK3 (NP_001092906.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | KCVA KKSL NKAS VENL LTEI EILK GIRH PHIV QLKD FQWD SDNI YLIM EFCA GGDL SRFI HTRR ILPE KVAR VFMQ QLAS ALQF LHER NISH LDLK PQNI LLSS LEKP HLKL ADFG FAQH MSPW DEKH VLRG SPLY MAPE MVCQ RQYD ARVD LWSM GVIL YEAL FGQP PFAS RSFS ELEE KIRS NRVI ELPL RPLL S |
| 遺伝子ID: | 25989 |
| Uniprot: | Q6PHR2 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 24kDa/53kDa/54kDa |
| 観察された分子量: | 60kDa |
| 同義語: | ULK3 |
| バックグラウンド: |
| UniProt Protein Function: | ULK3: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; ULK family Chromosomal Location of Human Ortholog: 15q24.1 Cellular Component: cytoplasm Molecular Function:protein binding; protein serine/threonine kinase activity Biological Process: autophagy; negative regulation of smoothened signaling pathway; positive regulation of smoothened signaling pathway; protein amino acid autophosphorylation |
| UniProt Code: | Q6PHR2 |
| NCBI GenInfo Identifier: | 259016166 |
| NCBI Gene ID: | 25989 |
| NCBI Accession: | Q6PHR2.2 |
| UniProt Secondary Accession: | Q6PHR2,Q9NPN5, Q9UFS4, B2RXK3, B4DFT0, B4DRQ7, D3DW68 |
| UniProt Related Accession: | Q6PHR2 |
| Molecular Weight: | 54,997 Da |
| NCBI Full Name: | Serine/threonine-protein kinase ULK3 |
| NCBI Synonym Full Names: | unc-51 like kinase 3 |
| NCBI Official Symbol: | ULK3 |
| NCBI Protein Information: | serine/threonine-protein kinase ULK3 |
| UniProt Protein Name: | Serine/threonine-protein kinase ULK3 |
| UniProt Synonym Protein Names: | Unc-51-like kinase 3 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | ULK3 |
| UniProt Entry Name: | ULK3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ULK3 antibody (CAB7587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
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