Anti-UBE2G2 Antibody (CAB10408)
- SKU:
- CAB10408
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-UBE2G2 Antibody |
| 抗体コード: | CAB10408 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human UBE2G2 (NP_003334.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, DU145, HT-1080, Mouse heart, Mouse testis, Rat heart, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human UBE2G2 (NP_003334.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAGT ALKR LMAE YKQL TLNP PEGI VAGP MNEE NFFE WEAL IMGP EDTC FEFG VFPA ILSF PLDY PLSP PKMR FTCE MFHP NIYP DGRV CISI LHAP GDDP MGYE SSAE RWSP VQSV EKIL LSVV SMLA EPND ESGA NVDA SKMW RDDR EQFY KIAK QIVQ KSLG L |
| 遺伝子ID: | 7327 |
| Uniprot: | P60604 |
| セルラーロケーション: | |
| 計算された分子量: | 15kDa/18kDa |
| 観察された分子量: | 19kDa |
| 同義語: | UBE2G2, UBC7 |
| バックグラウンド: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | UBE2G2: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Involved in endoplasmic reticulum- associated degradation (ERAD). Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; Endoplasmic reticulum; Ligase; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: cytosol Molecular Function:protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity Biological Process: cellular protein catabolic process; ER-associated protein catabolic process; protein amino acid N-linked glycosylation via asparagine; protein ubiquitination; ubiquitin-dependent protein catabolic process |
| NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
| UniProt Code: | P60604 |
| NCBI GenInfo Identifier: | 45593583 |
| NCBI Gene ID: | 7327 |
| NCBI Accession: | P60604.1 |
| UniProt Secondary Accession: | P60604,P56554, A6NMQ7, A8K3L4, D3DSL7, |
| UniProt Related Accession: | P60604 |
| Molecular Weight: | 19kDa |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 G2 |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 G2 |
| NCBI Official Symbol: | UBE2G2 |
| NCBI Official Synonym Symbols: | UBC7 |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 G2 |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 G2 |
| UniProt Synonym Protein Names: | E2 ubiquitin-conjugating enzyme G2; Ubiquitin carrier protein G2; Ubiquitin-protein ligase G2 |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2G2 |
 | Western blot analysis of extracts of various cell lines, using UBE2G2 antibody (CAB10408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of NIH-3T3 cells using UBE2G2 antibody (CAB10408) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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