Anti-TRPM8 Antibody (RACO0385)
- SKU:
- RACO0385
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | TRPM8 Antibody |
| Product SKU: | RACO0385 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, IHC |
| 推奨される希釈: | IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human TRPM8 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity. Plays a role in prostate cancer cell migration (PubMed:25559186). Isoform 2 and isoform 3 negatively regulate menthol- and cold-induced channel activity by stabilizing the closed state of the channel. |
| シノニム: | Transient receptor potential cation channel subfamily M member 8 (Long transient receptor potential channel 6) (LTrpC-6) (LTrpC6) (Transient receptor potential p8) (Trp-p8), TRPM8, LTRPC6 TRPP8 |
 | IHC image of RACO0385 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| UniProt Protein Function: | TRPM8: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Channel, cation; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 2q37.1 Cellular Component: plasma membrane Molecular Function:calcium channel activity; protein binding |
| UniProt Code: | Q7Z2W7 |
| NCBI GenInfo Identifier: | 143811469 |
| NCBI Gene ID: | 79054 |
| NCBI Accession: | Q7Z2W7.2 |
| UniProt Secondary Accession: | Q7Z2W7,Q3YFM7, Q6QNH9, Q8TAC3, Q8TDX8, Q9BVK1, A0AVG2 |
| UniProt Related Accession: | Q7Z2W7 |
| Molecular Weight: | 79,096 Da |
| NCBI Full Name: | Transient receptor potential cation channel subfamily M member 8 |
| NCBI Synonym Full Names: | transient receptor potential cation channel subfamily M member 8 |
| NCBI Official Symbol: | TRPM8 |
| NCBI Official Synonym Symbols: | TRPP8; LTRPC6 |
| NCBI Protein Information: | transient receptor potential cation channel subfamily M member 8 |
| UniProt Protein Name: | Transient receptor potential cation channel subfamily M member 8 |
| UniProt Synonym Protein Names: | Long transient receptor potential channel 6; LTrpC-6; LTrpC6; Transient receptor potential p8; Trp-p8 |
| Protein Family: | TRPM8 channel-associated factor |
| UniProt Gene Name: | TRPM8 |
| UniProt Entry Name: | TRPM8_HUMAN |