Anti-TAF5 Antibody (CAB7221)
- SKU:
- CAB7221
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-TAF5 Antibody |
| 抗体コード: | CAB7221 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 656-800 of human TAF5 (NP_008882.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 656-800 of human TAF5 (NP_008882.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VLNG NCVR IFTG HKGP IHSL TFSP NGRF LATG ATDG RVLL WDIG HGLM VGEL KGHT DTVC SLRF SRDG EILA SGSM DNTV RLWD AIKA FEDL ETDD FTTA TGHI NLPE NSQE LLLG TYMT KSTP VVHL HFTR RNLV LAAG AYSP Q |
| 遺伝子ID: | 6877 |
| Uniprot: | Q15542 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 80kDa/86kDa |
| 観察された分子量: | 100kDa |
| 同義語: | TAF5, TAF(II)100, TAF2D, TAFII-100, TAFII100 |
| バックグラウンド: | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | TAF5: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed. Belongs to the WD repeat TAF5 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 10q24-q25.2 Cellular Component: nucleoplasm; transcription factor TFIID complex; intracellular membrane-bound organelle; nucleolus; nucleus; actin cytoskeleton Molecular Function:protein dimerization activity; protein binding; histone acetyltransferase activity; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; regulation of transcription, DNA-dependent; transcription initiation; RNA elongation from RNA polymerase II promoter; gene expression; chromatin modification; histone acetylation |
| NCBI Summary: | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15542 |
| NCBI GenInfo Identifier: | 78103206 |
| NCBI Gene ID: | 6877 |
| NCBI Accession: | Q15542.3 |
| UniProt Secondary Accession: | Q15542,Q53EM4, Q5SYD5, Q86UZ7, Q9Y4K5, A8K5B4, B2RMR0 B7ZKJ6, |
| UniProt Related Accession: | Q15542 |
| Molecular Weight: | 800 |
| NCBI Full Name: | Transcription initiation factor TFIID subunit 5 |
| NCBI Synonym Full Names: | TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa |
| NCBI Official Symbol: | TAF5 |
| NCBI Official Synonym Symbols: | TAF2D; TAFII100 |
| NCBI Protein Information: | transcription initiation factor TFIID subunit 5; TAFII-100; TAF(II)100; TATA box binding protein (TBP)-associated factor 2D; transcription initiation factor TFIID 100 kD subunit; transcription initiation factor TFIID 100 kDa subunit; TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD |
| UniProt Protein Name: | Transcription initiation factor TFIID subunit 5 |
| UniProt Synonym Protein Names: | Transcription initiation factor TFIID 100 kDa subunit; TAF(II)100; TAFII-100; TAFII100 |
| Protein Family: | Transcription initiation factor |
| UniProt Gene Name: | TAF5 |
| UniProt Entry Name: | TAF5_HUMAN |
 | Western blot analysis of extracts of mouse brain, using TAF5 antibody (CAB7221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of A-549 cells using TAF5 antibody (CAB7221). Blue: DAPI for nuclear staining. |
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