Anti-SPK Antibody (CAB19947)[KO Validated]
![Anti-SPK Antibody (CAB19947)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/14421/14308/anti-spk-antibody-cab19947ko-validated__12171__46981.1645802658.jpg?c=1 "Anti-SPK Antibody (CAB19947)[KO Validated]")
- SKU:
- CAB19947
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
![Anti-SPK Antibody (CAB19947)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/14421/14308/anti-spk-antibody-cab19947ko-validated__12171__46981.1645802658.jpg?c=1&imbypass=on "Anti-SPK Antibody (CAB19947)[KO Validated]")
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Description
| 抗体名: | Anti-SPK Antibody [KO Validated] |
| 抗体コード: | CAB19947 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human SPK. |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T |
| 免疫原: | Recombinant protein of human SPK. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 55872 |
| Uniprot: | Q96KB5 |
| セルラーロケーション: | |
| 計算された分子量: | 36kDa/37kDa |
| 観察された分子量: | 36KDa |
![Anti-SPK Antibody [KO Validated] (CAB19947)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/d/292/A19947_1__42185.jpg) | Western blot analysis of extracts from normal (control) and SPK knockout (KO) 293T cells, using SPK antibody (CAB19947) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| 同義語: | PBK, CT84, HEL164, Nori-3, SPK, TOPK |
| バックグラウンド: | This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PBK: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Interacts with DLG1 and TP53. Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Activated by phosphorylation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily. |
| UniProt Protein Details: | Protein type:EC 2.7.12.2; Cancer Testis Antigen (CTA); Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; Other group; TOPK family Chromosomal Location of Human Ortholog: 8p21.2 Cellular Component: nucleus Molecular Function:ATP binding; protein binding; protein serine/threonine kinase activity Biological Process: mitosis; negative regulation of inflammatory response; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of stress-activated MAPK cascade; protein amino acid phosphorylation |
| NCBI Summary: | This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q96KB5 |
| NCBI GenInfo Identifier: | 83305809 |
| NCBI Gene ID: | 55872 |
| NCBI Accession: | Q96KB5.3 |
| UniProt Secondary Accession: | Q96KB5,Q9NPD9, Q9NYL7, Q9NZK6, B4DX68, D3DST2, |
| UniProt Related Accession: | Q96KB5 |
| Molecular Weight: | 37,211 Da |
| NCBI Full Name: | Lymphokine-activated killer T-cell-originated protein kinase |
| NCBI Synonym Full Names: | PDZ binding kinase |
| NCBI Official Symbol: | PBK |
| NCBI Official Synonym Symbols: | SPK; CT84; TOPK; HEL164; Nori-3 |
| NCBI Protein Information: | lymphokine-activated killer T-cell-originated protein kinase |
| UniProt Protein Name: | Lymphokine-activated killer T-cell-originated protein kinase |
| UniProt Synonym Protein Names: | Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase |
| Protein Family: | Lymphokine-activated killer T-cell-originated protein kinase |
| UniProt Gene Name: | PBK |
| UniProt Entry Name: | TOPK_HUMAN |