Anti-SOD1 Antibody (RACO0311)
- SKU:
- RACO0311
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- FC
- Conjugation:
- Unconjugated
Description
商品名: | SOD1 Antibody |
Product SKU: | RACO0311 |
サイズ: | 50ul |
宿主種: | Homo sapiens (Human) |
申し込み: | ELISA, WB, FC |
推奨される希釈: | WB:1:500-1:5000, FC:1:20-1:200 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human SOD1 |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Destroys radicals which are normally produced within the cells and which are toxic to biological systems. |
シノニム: | Superoxide dismutase [Cu-Zn] (EC 1.15.1.1) (Superoxide dismutase 1) (hSod1), SOD1 |
Overlay histogram showing Jurkat cells stained with RACO0311 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg) 1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg) 1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. | |
Western Blot. Positive WB detected in 293 whole cell lysate Hela whole cell lysate Jurkat whole cell lysate MCF-7 whole cell lysate K562 whole cell lysate HepG2 whole cell lysate U87 whole cell lysate All lanes SOD1 antibody at 1:1500. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 16 kDa. Observed band size: 18 kDa. |
UniProt Protein Function: | SOD1: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not. Belongs to the Cu-Zn superoxide dismutase family. |
UniProt Protein Details: | Protein type:Oxidoreductase; Mitochondrial; EC 1.15.1.1; Nuclear receptor co-regulator; Apoptosis Chromosomal Location of Human Ortholog: 21q22.11 Cellular Component: dendrite cytoplasm; extracellular space; protein complex; mitochondrion; extracellular region; mitochondrial intermembrane space; cytosol; nucleoplasm; extracellular matrix; cell soma; mitochondrial matrix; cytoplasm; plasma membrane; peroxisome; cytoplasmic vesicle; nucleus Molecular Function:identical protein binding; protein binding; protein homodimerization activity; copper ion binding; zinc ion binding; chaperone binding; superoxide dismutase activity; Rac GTPase binding; protein phosphatase 2B binding Biological Process: positive regulation of catalytic activity; activation of MAPK activity; positive regulation of apoptosis; cellular iron ion homeostasis; myeloid cell homeostasis; retrograde axon cargo transport; response to antibiotic; muscle maintenance; retinal homeostasis; glutathione metabolic process; regulation of mitochondrial membrane potential; neurofilament cytoskeleton organization and biogenesis; positive regulation of superoxide release; negative regulation of neuron apoptosis; placenta development; positive regulation of cytokine production; response to drug; platelet activation; cell aging; regulation of organ growth; transmission of nerve impulse; response to reactive oxygen species; response to ethanol; heart contraction; response to heat; superoxide release; relaxation of vascular smooth muscle; removal of superoxide radicals; locomotory behavior; response to organic substance; sensory perception of sound; platelet degranulation; ovarian follicle development; regulation of blood pressure; response to axon injury; auditory receptor cell stereocilium organization and biogenesis; anterograde axon cargo transport; negative regulation of cholesterol biosynthetic process; response to nutrient levels; response to superoxide; thymus development; regulation of T cell differentiation in the thymus; response to amphetamine; superoxide metabolic process; myelin maintenance in the peripheral nervous system; regulation of multicellular organism growth; response to hydrogen peroxide; response to copper ion; spermatogenesis; regulation of protein kinase activity; blood coagulation; embryo implantation; hydrogen peroxide biosynthetic process Disease: Amyotrophic Lateral Sclerosis 1 |
NCBI Summary: | The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | P00441 |
NCBI GenInfo Identifier: | 134611 |
NCBI Gene ID: | 6647 |
NCBI Accession: | P00441.2 |
UniProt Related Accession: | P00441 |
Molecular Weight: | |
NCBI Full Name: | Superoxide dismutase |
NCBI Synonym Full Names: | superoxide dismutase 1 |
NCBI Official Symbol: | SOD1 |
NCBI Official Synonym Symbols: | ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer |
NCBI Protein Information: | superoxide dismutase [Cu-Zn] |
UniProt Protein Name: | Superoxide dismutase [Cu-Zn] |
UniProt Synonym Protein Names: | Superoxide dismutase 1; hSod1 |
Protein Family: | Sodium channel |
UniProt Gene Name: | SOD1 |
UniProt Entry Name: | SODC_HUMAN |