Anti-SMYD4 Antibody (CAB2337)
- SKU:
- CAB2337
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SMYD4 Antibody |
| 抗体コード: | CAB2337 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF IP |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2). |
| 申し込み: | WB IF IP |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:20 - 1:50 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, HeLa, Jurkat |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MDLP VDEW KSYL LQKW ASLP TSVQ VTIS TAET LRDI FLHS SSLL QPED ELFL KRLS KGYL VGKD SDAP LFYR EEGN KKFQ EKDY TGAA VLYS KGVS HSRP NTED MSLC HANR SAAL FHLG QYET CLKD INRA QTHG YPER LQPK IMLR KAEC LVAL GRLQ EASQ TISD LERN FTAT PALA DVLP QTLQ RNLH RLKM KMQE KDSL TESF PAAL AKTL EDAA LREE NEQL SNAS SSIG LCVD PLKG RCLV ATKD ILPG ELLV |
| 遺伝子ID: | 114826 |
| Uniprot: | Q8IYR2 |
| セルラーロケーション: | |
| 計算された分子量: | 89kDa |
| 観察された分子量: | 90kDa |
| 同義語: | SMYD4, ZMYND21 |
| バックグラウンド: |
| UniProt Protein Function: | SMYD4: Belongs to the class V-like SAM-binding methyltransferase superfamily |
| UniProt Protein Details: | Protein type:Methyltransferase, protein lysine, predicted Chromosomal Location of Human Ortholog: 17p13.3 Molecular Function:methyltransferase activity; metal ion binding Biological Process: methylation |
| UniProt Code: | Q8IYR2 |
| NCBI GenInfo Identifier: | 296452956 |
| NCBI Gene ID: | 114826 |
| NCBI Accession: | Q8IYR2.3 |
| UniProt Secondary Accession: | Q8IYR2,Q8N1P2, Q8NAT0, Q96LV4, Q96PV2, |
| UniProt Related Accession: | Q8IYR2 |
| Molecular Weight: | 804 |
| NCBI Full Name: | SET and MYND domain-containing protein 4 |
| NCBI Synonym Full Names: | SET and MYND domain containing 4 |
| NCBI Official Symbol: | SMYD4 |
| NCBI Official Synonym Symbols: | ZMYND21 |
| NCBI Protein Information: | SET and MYND domain-containing protein 4 |
| UniProt Protein Name: | SET and MYND domain-containing protein 4 |
| Protein Family: | SET and MYND domain-containing protein |
| UniProt Gene Name: | SMYD4 |
| UniProt Entry Name: | SMYD4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SMYD4 antibody (CAB2337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of MCF-7 cells using SMYD4 antibody (CAB2337). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug SMYD4 antibody (CAB2337). Western blot was performed from the immunoprecipitate using SMYD4 antibody (CAB2337) at a dilition of 1:500.____Detection: ECL Enhanced Kit (RM00021)._Exposure time: 20s. |
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