Anti-SIX2 Antibody (CAB8727)
- SKU:
- CAB8727
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SIX2 Antibody |
| 抗体コード: | CAB8727 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human SIX2 (NP_058628.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:200 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | Rat kidney |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human SIX2 (NP_058628.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | PLNG SGKS VLGS SEDE KTPS GTPD HSSS SPAL LLSP PPPG LPSL HSLG HPPG PSAV PVPV PGGG GADP LQHH HGLQ DSIL NPMS ANLV DLGS |
| 遺伝子ID: | 10736 |
| Uniprot: | Q9NPC8 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 32kDa |
| 観察された分子量: | 32kDa |
| 同義語: | SIX2 |
| バックグラウンド: | This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. The encoded protein is a transcription factor which, like other members of this gene family, may be involved in limb or eye development. |
| UniProt Protein Function: | SIX2: Transcription factor that plays an important role in the development of several organs, including kidney, skull and stomach. During kidney development, maintains cap mesenchyme multipotent nephron progenitor cells in an undifferentiated state by opposing the inductive signals emanating from the ureteric bud and cooperates with WNT9B to promote renewing progenitor cells proliferation. Acts through its interaction with TCF7L2 and OSR1 in a canonical Wnt signaling independent manner preventing transcription of differentiation genes in cap mesenchyme such as WNT4. Also acts independently of OSR1 to activate expression of many cap mesenchyme genes, including itself, GDNF and OSR1. During craniofacial development plays a role in growth and elongation of the cranial base through regulation of chondrocyte differentiation. During stomach organogenesis, controls pyloric sphincter formation and mucosal growth through regulation of a gene network including NKX2-5, BMPR1B, BMP4, SOX9 and GREM1. During branchial arch development, acts to mediate HOXA2 control over the insulin-like growth factor pathway. Also may be involved in limb tendon and ligament development. Plays a role in cell proliferation and migration. {ECO:0000250|UniProtKB:Q62232, ECO:0000269|PubMed:22995329}. Belongs to the SIX/Sine oculis homeobox family. {ECO:0000305}. Interacts with TCF7L2; in a canonical Wnt signaling independent manner; prevents transcription of differentiation genes in cap mesenchyme. Interacts with OSR1; form a strong repressor complex with TCF7L2, TLE2 and TLE3 to prevent the activation of Wnt/beta-catenin target genes in the cap mesenchyme. Interacts with HOXA11, EYA1 and EYA3. {ECO:0000250|UniProtKB:Q62232} |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 2p21 Cellular Component: cytoplasm; nuclear membrane; nucleoplasm; nucleus; plasma membrane Biological Process: anatomical structure morphogenesis; anterior/posterior axis specification; cell migration; cell proliferation; embryonic digestive tract morphogenesis; kidney development; mesodermal cell fate specification |
| NCBI Summary: | This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. The encoded protein is a transcription factor which, like other members of this gene family, may be involved in limb or eye development. [provided by RefSeq, Dec 2008] |
| UniProt Code: | Q9NPC8 |
| NCBI GenInfo Identifier: | 12230598 |
| NCBI Gene ID: | 10736 |
| NCBI Accession: | Q9NPC8.1 |
| UniProt Secondary Accession: | Q9NPC8,Q9BXH7, |
| UniProt Related Accession: | Q9NPC8 |
| Molecular Weight: | 32,286 Da |
| NCBI Full Name: | Homeobox protein SIX2 |
| NCBI Synonym Full Names: | SIX homeobox 2 |
| NCBI Official Symbol: | SIX2 |
| NCBI Protein Information: | homeobox protein SIX2 |
| UniProt Protein Name: | Homeobox protein SIX2 |
| UniProt Synonym Protein Names: | Sine oculis homeobox homolog 2 |
| Protein Family: | Homeobox protein |
| UniProt Gene Name: | SIX2 |
| UniProt Entry Name: | SIX2_HUMAN |
 | Western blot analysis of extracts of Rat kidney, using SIX2 antibody (CAB8727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
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