Anti-SFI1 Antibody (CAB8360)
- SKU:
- CAB8360
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SFI1 Antibody |
| 抗体コード: | CAB8360 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 972-1211 of human SFI1 (NP_055590.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | MCF7 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 972-1211 of human SFI1 (NP_055590.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | HSAR KQPR RPHF LLEP AQSQ RPQK PQEH GLGM AQPA APSL TRPF LAEA PTAL VPHS PLPG ALSS APGP KQPP TAST GPEL LLLP LSSF MPCG AAAP ARVS AQRA TPRD KPPV PSSL ASVP DPHL LLPG DFSA TRAG PGLS TAGS LDLE AELE EIQQ QLLH YQTT KQNL WSCR RQAS SLRR WLEL NREE PGPE DQEV EQQV QKEL EQVE MQIQ LLAE ELQA QRQP IGAC VARI QALR QALC |
| 遺伝子ID: | 9814 |
| Uniprot: | A8K8P3 |
| セルラーロケーション: | Cytoplasm, centriole, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 117-147kDa |
| 観察された分子量: | 148kDa |
| 同義語: | SFI1, PISD, PPP1R139, hSfi1p |
| バックグラウンド: |
| UniProt Protein Function: | SFI1: Plays a role in the dynamic structure of centrosome- associated contractile fibers via its interaction with CETN2. Belongs to the SFI1 family. 7 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: centriole; cytosol Molecular Function:protein binding; phosphatase binding Biological Process: organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle |
| UniProt Code: | A8K8P3 |
| NCBI GenInfo Identifier: | 119580387 |
| NCBI Gene ID: | 9814 |
| NCBI Accession: | EAW59983.1 |
| UniProt Secondary Accession: | A8K8P3,O60289, Q2TAN8, A1L373, A1L387, A2A2L2, B1AKL9 B5MDB7, B7Z1V6, B7Z8G3, B7ZBE2, B7ZBE3, |
| UniProt Related Accession: | A8K8P3 |
| Molecular Weight: | |
| NCBI Full Name: | Sfi1 homolog, spindle assembly associated (yeast), isoform CRA_a |
| NCBI Synonym Full Names: | Sfi1 homolog, spindle assembly associated (yeast) |
| NCBI Official Symbol: | SFI1 |
| NCBI Official Synonym Symbols: | PISD; hSfi1p; PPP1R139 |
| NCBI Protein Information: | protein SFI1 homolog; homolog of yeast Sfi1; protein phosphatase 1, regulatory subunit 139 |
| UniProt Protein Name: | Protein SFI1 homolog |
| Protein Family: | Protein |
| UniProt Gene Name: | SFI1 |
| UniProt Entry Name: | SFI1_HUMAN |
 | Western blot analysis of extracts of MCF-7 cells, using SFI1 antibody (CAB8360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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