Anti-ROBO2 Antibody (CAB10070)
- SKU:
- CAB10070
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ROBO2 Antibody |
| 抗体コード: | CAB10070 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 450-660 of human ROBO2 (NP_001122401.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SH-SY5Y, SKOV3, A-549, Mouse brain, Mouse lung, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 450-660 of human ROBO2 (NP_001122401.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | TALL KCKA TGDP LPVI SWLK EGFT FPGR DPRA TIQE QGTL QIKN LRIS DTGT YTCV ATSS SGET SWSA VLDV TESG ATIS KNYD LSDL PGPP SKPQ VTDV TKNS VTLS WQPG TPGT LPAS AYII EAFS QSVS NSWQ TVAN HVKT TLYT VRGL RPNT IYLF MVRA INPQ GLSD PSPM SDPV RTQD ISPP AQGV DHRQ VQKE LGDV LVRL HNP |
| 遺伝子ID: | 6092 |
| Uniprot: | Q9HCK4 |
| セルラーロケーション: | Membrane, Single-pass type I membrane protein |
| 計算された分子量: | 130kDa/151kDa/153kDa |
| 観察された分子量: | 175kDa |
| 同義語: | ROBO2, SAX3 |
| バックグラウンド: | The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | RORB: Orphan nuclear receptor required for normal postnatal development of rod and cone photoreceptor cells. Regulates transcription of OPN1SW in cone photoreceptor cells by binding the sequence 5'-AGGTCA-3' in the OPN1SW promoter. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Nuclear receptor Chromosomal Location of Human Ortholog: 9q22 Cellular Component: nucleoplasm; nucleus Molecular Function:ligand-dependent nuclear receptor activity; protein binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; transcription factor binding Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; retinal rod cell development; positive regulation of transcription, DNA-dependent; rhythmic process; regulation of circadian rhythm; eye photoreceptor cell development; retinal cone cell development; visual perception; regulation of transcription, DNA-dependent; retina development in camera-type eye; gene expression; steroid hormone mediated signaling; negative regulation of osteoblast differentiation; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| UniProt Code: | Q9HCK4 |
| NCBI GenInfo Identifier: | 193083163 |
| NCBI Gene ID: | 6092 |
| NCBI Accession: | NP_001122401 |
| UniProt Secondary Accession: | Q9HCK4,Q9HCK4, |
| UniProt Related Accession: | Q9HCK4 |
| Molecular Weight: | 53 |
| NCBI Full Name: | roundabout homolog 2 isoform ROBO2a |
| NCBI Synonym Full Names: | roundabout guidance receptor 2 |
| NCBI Official Symbol: | ROBO2 |
| NCBI Official Synonym Symbols: | SAX3 |
| NCBI Protein Information: | roundabout homolog 2 |
| UniProt Protein Name: | Nuclear receptor ROR-beta |
| UniProt Synonym Protein Names: | Nuclear receptor RZR-beta; Nuclear receptor subfamily 1 group F member 2; Retinoid-related orphan receptor-beta |
| Protein Family: | Nuclear receptor |
| UniProt Gene Name: | RORB |
| UniProt Entry Name: | RORB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ROBO2 antibody (CAB10070) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
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