Anti-RHEB Antibody (RACO0505)
- SKU:
- RACO0505
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IF
- Application:
- ELISA
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | RHEB Antibody |
| Product SKU: | RACO0505 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, IF |
| 推奨される希釈: | IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human RHEB |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity. |
| シノニム: | GTP-binding protein Rheb (Ras homolog enriched in brain), RHEB, RHEB2 |
 | Immunofluorescence staining of Hela Cells with RACO0505 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| UniProt Protein Function: | RHEB: Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2. Ubiquitous. Highest levels observed in skeletal and cardiac muscle. Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2. Belongs to the small GTPase superfamily. Rheb family. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric; G protein, monomeric, Rheb Chromosomal Location of Human Ortholog: 7q36 Cellular Component: Golgi membrane; spliceosome; endoplasmic reticulum membrane; membrane; cell soma; dendrite; plasma membrane; cytosol Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; metal ion binding; protein kinase binding Biological Process: metabolic process; small GTPase mediated signal transduction; insulin receptor signaling pathway; signal transduction; cell cycle arrest; positive regulation of TOR signaling pathway |
| NCBI Summary: | This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15382 |
| NCBI GenInfo Identifier: | 6919957 |
| NCBI Gene ID: | 6009 |
| NCBI Accession: | Q15382.1 |
| UniProt Secondary Accession: | Q15382,Q53Y56, Q99444, B3KWN6, D3DX13, |
| UniProt Related Accession: | Q15382 |
| Molecular Weight: | 184 |
| NCBI Full Name: | GTP-binding protein Rheb |
| NCBI Synonym Full Names: | Ras homolog enriched in brain |
| NCBI Official Symbol: | RHEB |
| NCBI Official Synonym Symbols: | RHEB2 |
| NCBI Protein Information: | GTP-binding protein Rheb; Ras homolog enriched in brain 2 |
| UniProt Protein Name: | GTP-binding protein Rheb |
| UniProt Synonym Protein Names: | Ras homolog enriched in brain |
| Protein Family: | Rheb-like protein |
| UniProt Gene Name: | RHEB |
| UniProt Entry Name: | RHEB_HUMAN |