Anti-RGS20 Antibody (CAB8167)
- SKU:
- CAB8167
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | RGS20 Rabbit Polyclonal Antibody |
| 抗体コード: | CAB8167 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human RGS20 (NP_003693.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, NIH/3T3, SKOV3, HeLa, A-549, Mouse brain, Mouse spinal cord, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human RGS20 (NP_003693.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MRTA DGGE PAGA SSPA GRVD GGLQ MGSE RMEM RKRQ MPAA QDTP GAAP GQPG AGSR GSNA CCFC WCCC CSCS CLTV RNQE DQRP TIAS HELR ADLP TWEE SPAP TLEE VNAW AQSF DKLM VTPA GRNA FREF LRTE FSEE NMLF WMAC EELK KEAN KNII EEKA RIIY EDYI SILS PKEV SLDS RVRE VINR NMVE PSQH IFDD AQLQ IYTL MHRD SYPR FMNS AVYK DLLQ SLSE KSIE A |
| 遺伝子ID: | 8601 |
| Uniprot: | O76081 |
| セルラーロケーション: | Cytoplasm, Lipid-anchor, Membrane, Nucleus |
| 計算された分子量: | 17kDa/21kDa/25kDa/27kDa/31kDa/43kDa |
| 観察された分子量: | 44kDa |
| 同義語: | RGS20, RGSZ1, ZGAP1, g(z)GAP, gz-GAP |
| バックグラウンド: | The protein encoded by this gene belongs to the family of regulator of G protein signaling (RGS) proteins, which are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins inhibit signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound forms. This protein selectively binds to G(z)-alpha and G(alpha)-i2 subunits, and regulates their signaling activities. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | RGS20: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G- protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GAPs, misc.; GAPs Chromosomal Location of Human Ortholog: 8q11.23 Cellular Component: cytoplasm; plasma membrane; nucleus Molecular Function:protein binding; GTPase activator activity Biological Process: regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity |
| NCBI Summary: | The protein encoded by this gene belongs to the family of regulator of G protein signaling (RGS) proteins, which are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins inhibit signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound forms. This protein selectively binds to G(z)-alpha and G(alpha)-i2 subunits, and regulates their signaling activities. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | O76081 |
| NCBI GenInfo Identifier: | 18266795 |
| NCBI Gene ID: | 8601 |
| NCBI Accession: | O76081.4 |
| UniProt Secondary Accession: | O76081,Q96BG9, |
| UniProt Related Accession: | O76081 |
| Molecular Weight: | 27,060 Da |
| NCBI Full Name: | Regulator of G-protein signaling 20 |
| NCBI Synonym Full Names: | regulator of G-protein signaling 20 |
| NCBI Official Symbol: | RGS20 |
| NCBI Official Synonym Symbols: | RGSZ1; ZGAP1; gz-GAP; g(z)GAP |
| NCBI Protein Information: | regulator of G-protein signaling 20; regulator of G-protein signaling Z1; regulator of G-protein signalling 20; gz-selective GTPase-activating protein; regulator of G-protein signaling 20 variant 2; regulator of Gz-selective protein signaling 1 |
| UniProt Protein Name: | Regulator of G-protein signaling 20 |
| UniProt Synonym Protein Names: | Gz-selective GTPase-activating protein; G(z)GAP; Gz-GAP; Regulator of G-protein signaling Z1; Regulator of Gz-selective protein signaling 1 |
| Protein Family: | Regulator of G-protein signaling |
| UniProt Gene Name: | RGS20 |
| UniProt Entry Name: | RGS20_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RGS20 antibody (CAB8167) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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