Anti-RFX1 Antibody (CAB16066)
- SKU:
- CAB16066
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-RFX1 Antibody |
| 抗体コード: | CAB16066 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human RFX1 (NP_002909.4). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | LO2 |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human RFX1 (NP_002909.4). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QQVH SPPE QSPV QANS SSSK TAGA PTGT VPQQ LQVH GVQQ SVPV TQER SVVQ ATPQ APKP GPVQ PLTV QGLQ PVHV AQEV QQLQ QVPV PHVY SSQV QYVE G |
| 遺伝子ID: | 5989 |
| Uniprot: | P22670 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 104kDa |
| 観察された分子量: | 130kDa |
| 同義語: | RFX1, EFC, RFX |
| バックグラウンド: | This gene encodes a member of the regulatory factor X (RFX) family of transcription factors, which are characterized by a winged-helix DNA-binding domain. The encoded transcription factor contains an N-terminal activation domain and a C-terminal repression domain, and may activate or repress target gene expression depending on cellular context. This transcription factor has been shown to regulate a wide variety of genes involved in immunity and cancer, including the MHC class II genes and genes that may be involved in cancer progression. This gene exhibits altered expression in glioblastoma and the autoimmune disease systemic lupus erythematosis (SLE). |
| UniProt Protein Function: | RFX1: Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter. Belongs to the RFX family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus Molecular Function:protein binding; RNA polymerase II transcription factor activity, enhancer binding Biological Process: immune response |
| NCBI Summary: | This gene encodes a member of the regulatory factor X (RFX) family of transcription factors, which are characterized by a winged-helix DNA-binding domain. The encoded transcription factor contains an N-terminal activation domain and a C-terminal repression domain, and may activate or repress target gene expression depending on cellular context. This transcription factor has been shown to regulate a wide variety of genes involved in immunity and cancer, including the MHC class II genes and genes that may be involved in cancer progression. This gene exhibits altered expression in glioblastoma and the autoimmune disease systemic lupus erythematosis (SLE). [provided by RefSeq, Jul 2016] |
| UniProt Code: | P22670 |
| NCBI GenInfo Identifier: | 288558824 |
| NCBI Gene ID: | 5989 |
| NCBI Accession: | P22670.2 |
| UniProt Related Accession: | P22670 |
| Molecular Weight: | 104,758 Da |
| NCBI Full Name: | MHC class II regulatory factor RFX1 |
| NCBI Synonym Full Names: | regulatory factor X1 |
| NCBI Official Symbol: | RFX1 |
| NCBI Official Synonym Symbols: | EFC; RFX |
| NCBI Protein Information: | MHC class II regulatory factor RFX1 |
| UniProt Protein Name: | MHC class II regulatory factor RFX1 |
| UniProt Synonym Protein Names: | Enhancer factor C; EF-C; Regulatory factor X 1; RFX; Transcription factor RFX1 |
| Protein Family: | RFX-like DNA-binding protein |
| UniProt Gene Name: | RFX1 |
| UniProt Entry Name: | RFX1_HUMAN |
 | Western blot analysis of extracts of LO2 cells, using RFX1 antibody (CAB16066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
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