Anti-RFC4 Antibody (CAB5485)
- SKU:
- CAB5485
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-RFC4 Antibody |
| 抗体コード: | CAB5485 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF IP |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1). |
| 申し込み: | WB IHC IF IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | HeLa, SW480, 22Rv1, SW620, K-562, HepG2, MCF7 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DKIQ QQRL LDIA KKEN VKIS DEGI AYLV KVSE GDLR KAIT FLQS ATRL TGGK EITE KVIT DIAG VIPA EKID GVFA ACQS GSFD KLEA VVKD LIDE GHAA TQLV NQLH DVVV ENNL SDKQ KSII TEKL AEVD KCLA DGAD EHLQ LISL CATV MQQL SQNC |
| 遺伝子ID: | 5984 |
| Uniprot: | P35249 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 33kDa/39kDa |
| 観察された分子量: | 40kDa |
| 同義語: | RFC4, A1, RFC37 |
| バックグラウンド: | The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. |
| UniProt Protein Function: | RFC4: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. Belongs to the activator 1 small subunits family. |
| UniProt Protein Details: | Protein type:DNA replication Chromosomal Location of Human Ortholog: 3q27 Cellular Component: nucleoplasm; DNA replication factor C complex; cytoplasm; nucleus Molecular Function:protein binding; ATP binding; DNA clamp loader activity Biological Process: telomere maintenance via semi-conservative replication; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; DNA strand elongation during DNA replication; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; DNA repair; telomere maintenance |
| NCBI Summary: | The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35249 |
| NCBI GenInfo Identifier: | 1703052 |
| NCBI Gene ID: | 5984 |
| NCBI Accession: | P35249.2 |
| UniProt Secondary Accession: | P35249,Q6FHX7, B4DM41, D3DNV2, |
| UniProt Related Accession: | P35249 |
| Molecular Weight: | 363 |
| NCBI Full Name: | Replication factor C subunit 4 |
| NCBI Synonym Full Names: | replication factor C (activator 1) 4, 37kDa |
| NCBI Official Symbol: | RFC4 |
| NCBI Official Synonym Symbols: | A1; RFC37 |
| NCBI Protein Information: | replication factor C subunit 4; A1 37 kDa subunit; RFC 37 kDa subunit; RF-C 37 kDa subunit; activator 1 subunit 4; activator 1 37 kDa subunit; replication factor C 37 kDa subunit |
| UniProt Protein Name: | Replication factor C subunit 4 |
| UniProt Synonym Protein Names: | Activator 1 37 kDa subunit; A1 37 kDa subunit; Activator 1 subunit 4; Replication factor C 37 kDa subunit; RF-C 37 kDa subunit; RFC37 |
| Protein Family: | Replication factor |
| UniProt Gene Name: | RFC4 |
| UniProt Entry Name: | RFC4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RFC4 antibody (CAB5485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (CAB5485) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (CAB5485) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (CAB5485) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using RFC4 antibody (CAB5485). Blue: DAPI for nuclear staining. |
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