Anti-RET Antibody (RACO0270)
- SKU:
- RACO0270
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | RET Antibody |
| Product SKU: | RACO0270 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, FC |
| 推奨される希釈: | FC:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Ret |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. |
| シノニム: | Proto-oncogene tyrosine-protein kinase receptor Ret (EC 2.7.10.1) (Cadherin family member 12) (Proto-oncogene c-Ret) [Cleaved into: Soluble RET kinase fragment, Extracellular cell-membrane anchored RET cadherin 120 kDa fragment], RET, CDHF12 CDHR16 PTC RET51 |
 | Overlay histogram showing Hela cells stained with RACO0270 (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg) 1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg) 1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| UniProt Protein Function: | Ret: a proto-oncogenic receptor tyrosine kinase. Receptor for glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Part of a multicompetent receptor complex with other membrane-bound ligand-binding GDNF family receptors (aGFRs). Required for development of the kidney and neural crest-derived cell types. Three alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Protein kinase, TK; EC 2.7.10.1; Oncoprotein; Kinase, protein; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; Ret family Chromosomal Location of Human Ortholog: 10q11.2 Cellular Component: cytoplasm; endosome membrane; integral to plasma membrane; intracellular membrane-bound organelle; plasma membrane; receptor complex Molecular Function:calcium ion binding; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor activity Biological Process: MAPKKK cascade; membrane protein proteolysis; neuron adhesion; positive regulation of cell adhesion mediated by integrin; positive regulation of cell migration; positive regulation of transcription, DNA-dependent; posterior midgut development; protein amino acid phosphorylation; regulation of cell adhesion; response to pain; signal transduction Disease: Hirschsprung Disease, Susceptibility To, 1; Multiple Endocrine Neoplasia, Type Iia; Multiple Endocrine Neoplasia, Type Iib; Thyroid Carcinoma, Familial Medullary; Thyroid Carcinoma, Papillary |
| NCBI Summary: | This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P07949 |
| NCBI GenInfo Identifier: | 547807 |
| NCBI Gene ID: | 5979 |
| NCBI Accession: | P07949.3 |
| UniProt Secondary Accession: | P07949,Q15250, Q9BTB0, Q9H4A2, A8K6Z2, |
| UniProt Related Accession: | P07949 |
| Molecular Weight: | 119,847 Da |
| NCBI Full Name: | Proto-oncogene tyrosine-protein kinase receptor Ret |
| NCBI Synonym Full Names: | ret proto-oncogene |
| NCBI Official Symbol: | RET |
| NCBI Official Synonym Symbols: | PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1 |
| NCBI Protein Information: | proto-oncogene tyrosine-protein kinase receptor Ret |
| UniProt Protein Name: | Proto-oncogene tyrosine-protein kinase receptor Ret |
| UniProt Synonym Protein Names: | Cadherin family member 12; Proto-oncogene c-Ret |
| Protein Family: | Retbindin |
| UniProt Gene Name: | RET |
| UniProt Entry Name: | RET_HUMAN |