Anti-RAP2B Antibody (CAB4071)
- SKU:
- CAB4071
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-RAP2B Antibody |
| 抗体コード: | CAB4071 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human RAP2B (NP_002877.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:100 - 1:500 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse brain, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human RAP2B (NP_002877.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MREY KVVV LGSG GVGK SALT VQFV TGSF IEKY DPTI EDFY RKEI EVDS SPSV LEIL DTAG TEQF ASMR DLYI KNGQ GFIL VYSL VNQQ SFQD IKPM RDQI IRVK RYER VPMI LVGN KVDL EGER EVSY GEGK ALAE EWSC PFME TSAK NKAS VDEL FAEI VRQM NYAA QPNG DEGC CSAC VIL |
| 遺伝子ID: | 5912 |
| Uniprot: | P61225 |
| セルラーロケーション: | Cytoplasmic side, Lipid-anchor, Recycling endosome membrane |
| 計算された分子量: | 20kDa |
| 観察された分子量: | 18kDa |
| 同義語: | RAP2B |
| バックグラウンド: | This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. |
| UniProt Protein Function: | RAP2B: Small GTP-binding protein which cycles between a GDP- bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells. Belongs to the small GTPase superfamily. Ras family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Ras; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 3q25.2 Cellular Component: tight junction; membrane; recycling endosome membrane; plasma membrane; cytosol; lipid raft Molecular Function:protein domain specific binding; GDP binding; GTP binding Biological Process: platelet activation; microvillus biogenesis; signal transduction; positive regulation of protein amino acid autophosphorylation; negative regulation of cell migration; Rap protein signal transduction |
| NCBI Summary: | This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61225 |
| NCBI GenInfo Identifier: | 47117762 |
| NCBI Gene ID: | 5912 |
| NCBI Accession: | P61225.1 |
| UniProt Secondary Accession: | P61225,P17964, Q96EG5, Q9CXG0, |
| UniProt Related Accession: | P61225 |
| Molecular Weight: | 183 |
| NCBI Full Name: | Ras-related protein Rap-2b |
| NCBI Synonym Full Names: | RAP2B, member of RAS oncogene family |
| NCBI Official Symbol: | RAP2B |
| NCBI Protein Information: | ras-related protein Rap-2b; small GTP binding protein |
| UniProt Protein Name: | Ras-related protein Rap-2b |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAP2B |
| UniProt Entry Name: | RAP2B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RAP2B antibody (CAB4071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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