Anti-RAD9A Antibody (CAB1890)
- SKU:
- CAB1890
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-RAD9A Antibody |
| 抗体コード: | CAB1890 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 162-391 of human RAD9A (NP_004575.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | BT-474, 22Rv1, HepG2, MCF7 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 162-391 of human RAD9A (NP_004575.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ALAE VTLG IGRG RRVI LRSY HEEE ADST AKAM VTEM CLGE EDFQ QLQA QEGV AITF CLKE FRGL LSFA ESAN LNLS IHFD APGR PAIF TIKD SLLD GHFV LATL SDTD SHSQ DLGS PERH QPVP QLQA HSTP HPDD FAND DIDS YMIA METT IGNE GSRV LPSI SLSP GPQP PKSP GPHS EEED EAEP STVP GTPP PKKF RSLF FGSI LAPV RSPQ GPSP VLAE DSEG EG |
| 遺伝子ID: | 5883 |
| Uniprot: | Q99638 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 42kDa |
| 観察された分子量: | 60kDa |
| 同義語: | RAD9A, RAD9 |
| バックグラウンド: | This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Rad9: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9. |
| UniProt Protein Details: | Protein type:EC 3.1.11.2; DNA replication; Deoxyribonuclease; Apoptosis; Cell cycle regulation Chromosomal Location of Human Ortholog: 11q13.1-q13.2 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein binding; enzyme binding; histone deacetylase binding; exodeoxyribonuclease III activity; 3'-5' exonuclease activity; SH3 domain binding; protein kinase binding Biological Process: intra-S DNA damage checkpoint; DNA damage checkpoint; DNA replication checkpoint; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; response to DNA damage stimulus |
| NCBI Summary: | This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q99638 |
| NCBI GenInfo Identifier: | 74717382 |
| NCBI Gene ID: | 5883 |
| NCBI Accession: | Q99638.1 |
| UniProt Related Accession: | Q99638 |
| Molecular Weight: | |
| NCBI Full Name: | Cell cycle checkpoint control protein RAD9A |
| NCBI Synonym Full Names: | RAD9 checkpoint clamp component A |
| NCBI Official Symbol: | RAD9A |
| NCBI Official Synonym Symbols: | RAD9 |
| NCBI Protein Information: | cell cycle checkpoint control protein RAD9A |
| UniProt Protein Name: | Cell cycle checkpoint control protein RAD9A |
| UniProt Synonym Protein Names: | DNA repair exonuclease rad9 homolog A |
| Protein Family: | RAD9, HUS1, RAD1-interacting nuclear orphan protein |
| UniProt Gene Name: | RAD9A |
| UniProt Entry Name: | RAD9A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RAD9A antibody (CAB1890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
 | Immunohistochemistry of paraffin-embedded rat liver using RAD9A antibody (CAB1890) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver damage using RAD9A antibody (CAB1890) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of MCF-7 cells using RAD9A antibody (CAB1890). Blue: DAPI for nuclear staining. |
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