Anti-PUS10 Antibody (CAB15962)
- SKU:
- CAB15962
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-PUS10 Antibody |
| 抗体コード: | CAB15962 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse thymus, Mouse spleen, Mouse testis, Rat thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VAGR YNKY SRNL PQTP WIID GERK LESS VEEL ISDH LLAV FKAE SFNF SSSG REDV DVRT LGNG RPFA IELV NPHR VHFT SQEI KELQ QKIN NSSN KIQV RDLQ LVTR EAIG HMKE GEEE KTKT YSAL IWTN KAIQ KKDI EFLN DIKD LKID QKTP LRVL HRRP LAVR ARVI HFME TQYV DEHH FRLH LKTQ AGTY IKEF VHGD FGRT KPNI GSLM NVTA DILE LDVE SVDV DWPP ALDD |
| 遺伝子ID: | 150962 |
| Uniprot: | Q3MIT2 |
| セルラーロケーション: | |
| 計算された分子量: | 60kDa |
| 観察された分子量: | 60kDa |
| 同義語: | PUS10, CCDC139, DOBI |
| バックグラウンド: | Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]). |
| UniProt Protein Function: | PUS10: Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death. Belongs to the pseudouridine synthase Pus10 family. |
| UniProt Protein Details: | Protein type:RNA processing; Isomerase; EC 5.4.99.- Chromosomal Location of Human Ortholog: 2p16.1 Molecular Function:RNA binding; pseudouridine synthase activity Biological Process: pseudouridine synthesis; tRNA processing |
| NCBI Summary: | Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009] |
| UniProt Code: | Q3MIT2 |
| NCBI GenInfo Identifier: | 207113130 |
| NCBI Gene ID: | 150962 |
| NCBI Accession: | NP_653310.2 |
| UniProt Secondary Accession: | Q3MIT2,Q5JPJ5, Q96MI8, |
| UniProt Related Accession: | Q3MIT2 |
| Molecular Weight: | 60,244 Da |
| NCBI Full Name: | putative tRNA pseudouridine synthase Pus10 |
| NCBI Synonym Full Names: | pseudouridylate synthase 10 |
| NCBI Official Symbol: | PUS10 |
| NCBI Official Synonym Symbols: | DOBI; CCDC139 |
| NCBI Protein Information: | putative tRNA pseudouridine synthase Pus10 |
| UniProt Protein Name: | Putative tRNA pseudouridine synthase Pus10 |
| UniProt Synonym Protein Names: | Coiled-coil domain-containing protein 139; tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase |
| Protein Family: | Putative tRNA pseudouridine synthase |
| UniProt Gene Name: | PUS10 |
| UniProt Entry Name: | PUS10_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PUS10 antibody (CAB15962) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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