Anti-PTGS1 Antibody (RACO0252)
- SKU:
- RACO0252
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | PTGS1 Antibody |
| Product SKU: | RACO0252 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, FC |
| 推奨される希釈: | WB:1:500-1:5000, FC:1:20-1:200 |
| 反応性: | Human, Mouse |
| 免疫原: | A synthesized peptide derived from human COX1 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells. |
| シノニム: | Prostaglandin G/H synthase 1 (EC 1.14.99.1) (Cyclooxygenase-1) (COX-1) (Prostaglandin H2 synthase 1) (PGH synthase 1) (PGHS-1) (PHS 1) (Prostaglandin-endoperoxide synthase 1), PTGS1, COX1 |
 | Overlay histogram showing Hela cells stained with RACO0252 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg) 1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg) 1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
 | Western Blot. Positive WB detected in HL60 whole cell lysate SH-SY5Y whole cell lysate Mouse liver tissue. All lanes PTGS1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 69, 65, 62, 57, 72, 73 kDa. Observed band size: 72 kDa. |
| UniProt Protein Function: | COX-1: May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. Homodimer. Belongs to the prostaglandin G/H synthase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.14.99.1; Lipid Metabolism - arachidonic acid; Oxidoreductase Chromosomal Location of Human Ortholog: 9q32-q33.3 Cellular Component: endoplasmic reticulum membrane; photoreceptor outer segment; intracellular membrane-bound organelle; cytoplasm; nucleus Molecular Function:prostaglandin-endoperoxide synthase activity; peroxidase activity; dioxygenase activity; metal ion binding; heme binding Biological Process: regulation of blood pressure; xenobiotic metabolic process; cyclooxygenase pathway; arachidonic acid metabolic process; response to oxidative stress; lipid metabolic process; prostaglandin biosynthetic process; inflammatory response; regulation of cell proliferation |
| NCBI Summary: | This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| UniProt Code: | P23219 |
| NCBI GenInfo Identifier: | 317373262 |
| NCBI Gene ID: | 5742 |
| NCBI Accession: | P23219.2 |
| UniProt Secondary Accession: | P23219,Q15122, Q3HY28, Q3HY29, Q5T7T6, Q5T7T7, Q5T7T8 A8K1V7, B4DHQ2, B4E2S5, |
| UniProt Related Accession: | P23219 |
| Molecular Weight: | 72,010 Da |
| NCBI Full Name: | Prostaglandin G/H synthase 1 |
| NCBI Synonym Full Names: | prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) |
| NCBI Official Symbol: | PTGS1 |
| NCBI Official Synonym Symbols: | COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1 |
| NCBI Protein Information: | prostaglandin G/H synthase 1; PGH synthase 1; cyclooxygenase-1; prostaglandin H2 synthase 1 |
| UniProt Protein Name: | Prostaglandin G/H synthase 1 |
| UniProt Synonym Protein Names: | Cyclooxygenase-1; COX-1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; Prostaglandin-endoperoxide synthase 1 |
| Protein Family: | Cox1 intron-like protein |
| UniProt Gene Name: | PTGS1 |
| UniProt Entry Name: | PGH1_HUMAN |