Anti-PSEN2 Antibody (CAB7719)
- SKU:
- CAB7719
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| 抗体名: | Anti-PSEN2 Antibody |
| 抗体コード: | CAB7719 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human PSEN2. |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SH-SY5Y, Mouse brain, Rat brain |
| 免疫原: | Recombinant protein of human PSEN2. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 5664 |
| Uniprot: | P49810 |
| セルラーロケーション: | Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein |
| 計算された分子量: | 46kDa/50kDa |
| 観察された分子量: | 21KDa/35KDa/50KDa |
| 同義語: | PSEN2, AD3L, AD4, CMD1V, PS2, STM2 |
| バックグラウンド: | Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. |
| UniProt Protein Function: | PSEN2: probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). An integral membrane protein of the Golgi and endoplasmic reticulum. Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. Two alternative splice isoforms have been identified. |
| UniProt Protein Details: | Protein type:EC 3.4.23.-; Membrane protein, integral; Membrane protein, multi-pass; Cell surface; Protease Chromosomal Location of Human Ortholog: 1q42.13 Cellular Component: Golgi apparatus; centrosome; cell surface; integral to plasma membrane; lysosomal membrane; nuclear inner membrane; cytosol; lipid raft; ciliary rootlet; kinetochore; cell soma; membrane; perinuclear region of cytoplasm; apical plasma membrane; mitochondrial inner membrane; dendritic shaft; neuromuscular junction; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; cell cortex; Z disc; Golgi membrane; growth cone; axon; plasma membrane Molecular Function:protein binding; endopeptidase activity; aspartic-type endopeptidase activity Biological Process: positive regulation of catalytic activity; axon guidance; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; protein maturation; regulation of synaptic plasticity; positive regulation of coagulation; cell fate specification; T cell receptor signaling pathway; T cell activation during immune response; protein transport; amyloid precursor protein catabolic process; hair follicle development; negative regulation of protein amino acid phosphorylation; beta-amyloid metabolic process; calcium ion transport; forebrain development; ephrin receptor signaling pathway; brain morphogenesis; negative regulation of protein binding; dorsoventral neural tube patterning; embryonic limb morphogenesis; cardiac muscle contraction; Notch signaling pathway; somitogenesis; membrane protein intracellular domain proteolysis; thymus development; regulation of epidermal growth factor receptor activity; membrane protein ectodomain proteolysis; Notch receptor processing; memory; endoplasmic reticulum calcium ion homeostasis; myeloid leukocyte differentiation; response to hypoxia; hemopoietic progenitor cell differentiation; protein processing; negative regulation of protein complex assembly; alveolus development; negative regulation of apoptosis Disease: Alzheimer Disease 4; Cardiomyopathy, Dilated, 1v |
| NCBI Summary: | Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49810 |
| NCBI GenInfo Identifier: | 1709858 |
| NCBI Gene ID: | 5664 |
| NCBI Accession: | P49810.1 |
| UniProt Secondary Accession: | P49810,Q96P32, A8K8D4, B1AP21, |
| UniProt Related Accession: | P49810 |
| Molecular Weight: | 50,011 Da |
| NCBI Full Name: | Presenilin-2 |
| NCBI Synonym Full Names: | presenilin 2 |
| NCBI Official Symbol: | PSEN2 |
| NCBI Official Synonym Symbols: | AD4; PS2; AD3L; STM2; CMD1V |
| NCBI Protein Information: | presenilin-2; AD5; E5-1; PS-2; AD3LP; STM-2; Alzheimer disease 4 |
| UniProt Protein Name: | Presenilin-2 |
| UniProt Synonym Protein Names: | AD3LP; AD5; E5-1; STM-2Cleaved into the following 2 chains:Presenilin-2 NTF subunit; Presenilin-2 CTF subunit |
| Protein Family: | Presenilin |
| UniProt Gene Name: | PSEN2 |
| UniProt Entry Name: | PSN2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PSEN2 antibody (CAB7719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat heart using PSEN2 antibody (CAB7719) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human kidney using PSEN2 antibody (CAB7719) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using PSEN2 Rabbit pAb (CAB7719) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |