Anti-PRPF18 Antibody (CAB12817)
- SKU:
- CAB12817
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-PRPF18 Antibody |
| 抗体コード: | CAB12817 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human PRPF18 (NP_003666.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | HeLa |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human PRPF18 (NP_003666.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MDIL KSEI LRKR QLVE DRNL LVEN KKYF KRSE LAKK EEEA YFER CGYK IQPK EEDQ KPLT SSNP VLEL ELAE EKLP MTLS RQEV IRRL RERG EPIR LFGE TDYD AFQR LRKI EILT PEVN KGLR NDLK AALD KIDQ QYLN EIVG GQEP GEED TQND LKVH EENT TIEE LEAL GESL GKGD DHKD MDII TKFL KFLL GVWA KELN ARED YVKR SVQG KLNS ATQK QTES YLRP LFRK LRKR NLPA DIKE SITD IIKF MLQR EYVK ANDA YLQM AIGN APWP IGVT MVGI HART GREK IFSK HVAH VLND ETQR KYIQ GLKR LMTI CQKH FPTD PSKC VEYN AL |
| 遺伝子ID: | 8559 |
| Uniprot: | Q99633 |
| セルラーロケーション: | Nucleus speckle |
| 計算された分子量: | 5kDa/39kDa |
| 観察された分子量: | 38kDa |
| 同義語: | PRPF18, PRP18, hPrp18 |
| バックグラウンド: | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. |
| UniProt Protein Function: | PRPF18: Participates in the second step of pre-mRNA splicing. Belongs to the PRP18 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA splicing; Spliceosome Chromosomal Location of Human Ortholog: 10p13 Cellular Component: nucleus; snRNP U5; spliceosome; U4/U6 x U5 tri-snRNP complex Molecular Function:second spliceosomal transesterification activity Biological Process: generation of catalytic spliceosome for second transesterification step; mRNA processing; RNA splicing |
| NCBI Summary: | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99633 |
| NCBI GenInfo Identifier: | 37082244 |
| NCBI Gene ID: | 8559 |
| NCBI Accession: | Q99633.1 |
| UniProt Secondary Accession: | Q99633,Q5T9P9, Q9BUI9, |
| UniProt Related Accession: | Q99633 |
| Molecular Weight: | 40kDa |
| NCBI Full Name: | Pre-mRNA-splicing factor 18 |
| NCBI Synonym Full Names: | pre-mRNA processing factor 18 |
| NCBI Official Symbol: | PRPF18 |
| NCBI Official Synonym Symbols: | PRP18; hPrp18 |
| NCBI Protein Information: | pre-mRNA-splicing factor 18 |
| UniProt Protein Name: | Pre-mRNA-splicing factor 18 |
| UniProt Synonym Protein Names: | PRP18 homolog; hPRP18 |
| UniProt Gene Name: | PRPF18 |
 | Western blot analysis of extracts of HeLa cells, using PRPF18 antibody (CAB12817) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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