Anti-PRMT1 Antibody (CAB1055)
- SKU:
- CAB1055
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PRMT1 Antibody |
| 抗体コード: | CAB1055 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-353 of human PRMT1 (NP_938074.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, A549, rat spleen, rat lung, rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-353 of human PRMT1 (NP_938074.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | SSEK PNAE DMTS KDYY FDSY AHFG IHEE MLKD EVRT LTYR NSMF HNRH LFKD KVVL DVGS GTGI LCMF AAKA GARK VIGI ECSS ISDY AVKI VKAN KLDH VVTI IKGK VEEV ELPV EKVD IIIS EWMG YCLF YESM LNTV LYAR DKWL APDG LIFP DRAT LYVT AIED RQYK DYKI HWWE NVYG FDMS CIKD VAIK EPLV DVVD PKQL VTNA CLIK EVDI YTVK VEDL TFTS PFCL QVKR NDYV HALV AYFN IEFT RCHK RTGF STSP ESPY THWK QTVF YMED YLTV KTGE EIFG TIGM RPNA KNNR DLDF TIDL DFKG QLCE LSCS TDYR MR |
| 遺伝子ID: | 3276 |
| Uniprot: | Q99873 |
| セルラーロケーション: | Cytoplasm, Nucleus, cytosol, nucleoplasm |
| 計算された分子量: | 39kDa/40kDa/41kDa |
| 観察された分子量: | 42kDa |
| 同義語: | PRMT1, ANM1, HCP1, HRMT1L2, IR1B4 |
| バックグラウンド: | This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. |
| UniProt Protein Function: | PRMT1: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Homodimer and heterodimer with PRMT8. The dimer can then associate to form a homohexamer. Interacts with ILF3, BTG1, BTG2, SUPT5H and interferon-alpha/beta receptor 1. Interacts with NFATC2IP. Widely expressed. By BTG1, BTG2 and ILF3. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Methyltransferase; EC 2.1.1.125; Nuclear receptor co-regulator; Methyltransferase, protein arginine Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:identical protein binding; protein-arginine omega-N asymmetric methyltransferase activity; histone methyltransferase activity; methyltransferase activity; protein binding; N-methyltransferase activity Biological Process: histone methylation; peptidyl-arginine methylation; establishment and/or maintenance of chromatin architecture; cell surface receptor linked signal transduction; protein amino acid methylation; regulation of transcription, DNA-dependent; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of megakaryocyte differentiation; neurite development |
| NCBI Summary: | This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q99873 |
| NCBI GenInfo Identifier: | 161789011 |
| NCBI Gene ID: | 3276 |
| NCBI Accession: | Q99873.2 |
| UniProt Secondary Accession: | Q99873,Q15529, Q2VP93, Q6LEU5, Q8WUW5, Q99872, Q99874 Q9NZ04, Q9NZ05, Q9NZ06, B4E3C3, G5E9B6, |
| UniProt Related Accession: | Q99873 |
| Molecular Weight: | 361 |
| NCBI Full Name: | Protein arginine N-methyltransferase 1 |
| NCBI Synonym Full Names: | protein arginine methyltransferase 1 |
| NCBI Official Symbol: | PRMT1 |
| NCBI Official Synonym Symbols: | ANM1; HCP1; IR1B4; HRMT1L2 |
| NCBI Protein Information: | protein arginine N-methyltransferase 1; interferon receptor 1-bound protein 4; histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2 |
| UniProt Protein Name: | Protein arginine N-methyltransferase 1 |
| UniProt Synonym Protein Names: | Histone-arginine N-methyltransferase PRMT1 (EC:2.1.1.125); Interferon receptor 1-bound protein 4 |
| Protein Family: | Protein arginine N-methyltransferase |
| UniProt Gene Name: | PRMT1 |
| UniProt Entry Name: | ANM1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PRMT1 antibody (CAB1055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using PRMT1 antibody (CAB1055) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using PRMT1 antibody (CAB1055) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using PRMT1 antibody (CAB1055) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using PRMT1 Rabbit pAb (CAB1055) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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