Anti-PPL Antibody (CAB14051)
- SKU:
- CAB14051
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PPL Antibody |
| 抗体コード: | CAB14051 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPL (NP_002696.3). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse lung, Mouse heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPL (NP_002696.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MNSL FRKR NKGK YSPT VQTR SISN KELS ELIE QLQK NADQ VEKN IVDT EAKM QSDL ARLQ EGRQ PEHR DVTL QKVL DSEK LLYV LEAD AAIA KHMK HPQG DMIA EDIR QLKE RVTN LRGK HKQI YRLA VK |
| 遺伝子ID: | 5493 |
| Uniprot: | O60437 |
| セルラーロケーション: | Cell junction, Cell membrane, Cytoplasm, Mitochondrion, Nucleus, cytoskeleton, desmosome |
| 計算された分子量: | 204kDa |
| 観察された分子量: | 205kDa |
| 同義語: | PPL |
| バックグラウンド: | The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. |
| UniProt Protein Function: | periplakin: Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling. Belongs to the plakin or cytolinker family. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: desmosome; cytoskeleton; mitochondrion; plasma membrane; nucleus Molecular Function:protein binding; structural constituent of cytoskeleton Biological Process: keratinization |
| NCBI Summary: | The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O60437 |
| NCBI GenInfo Identifier: | 317373534 |
| NCBI Gene ID: | 5493 |
| NCBI Accession: | O60437.4 |
| UniProt Related Accession: | O60437 |
| Molecular Weight: | ~ 205kDa |
| NCBI Full Name: | Periplakin |
| NCBI Synonym Full Names: | periplakin |
| NCBI Official Symbol: | PPL |
| NCBI Protein Information: | periplakin |
| UniProt Protein Name: | Periplakin |
| UniProt Synonym Protein Names: | 190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope precursor protein |
| Protein Family: | Periplakin |
| UniProt Gene Name: | PPL |
| UniProt Entry Name: | PEPL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPL Rabbit pAb (CAB14051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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